Estrogens enhances the number of dopamine neurons of adult male mice though upregulating neurotrophic factors

伊宏亮; 郭文琼; 孙卫忠; 郭亮; 黄伟; 范晓棠; 徐海伟

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Estrogens enhances the number of dopamine neurons of adult male mice though upregulating neurotrophic factors

更新时间：2026-01-09

- Estrogens enhances the number of dopamine neurons of adult male mice though upregulating neurotrophic factors
- Journal of Army Medical University Vol. 33, Issue 7, Pages: 663-666(2011)
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  CLC： Q591.9;R322.81;R329.24
- Published：2011
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伊宏亮,郭文琼,孙卫忠,郭亮,黄伟,范晓棠,徐海伟.雌激素上调神经营养因子增加雄性小鼠中脑多巴胺神经元数量的实验研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):663-666.

　Yi Hongliang,Guo Wenqiong,Sun Weizhong,Guo Liang,Huang Wei,Fan Xiaotang,Xu Haiwei.Estrogens enhances the number of dopamine neurons of adult male mice though upregulating neurotrophic factors[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):663-666.
伊宏亮,郭文琼,孙卫忠,郭亮,黄伟,范晓棠,徐海伟.雌激素上调神经营养因子增加雄性小鼠中脑多巴胺神经元数量的实验研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):663-666. DOI：

　Yi Hongliang,Guo Wenqiong,Sun Weizhong,Guo Liang,Huang Wei,Fan Xiaotang,Xu Haiwei.Estrogens enhances the number of dopamine neurons of adult male mice though upregulating neurotrophic factors[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):663-666. DOI：

摘要

目的观察雌激素处理对成年雄性小鼠黑质多巴胺神经元数量的影响及可能的调控机制。方法选用雄性C57/BL6小鼠10只，分成对照组、雌激素组(E

)，采用免疫组化检测雌激素处理对小鼠黑质多巴胺神经元与calbindin阳性神经元数量的改变；采用RT-PCR分析雌激素处理对小鼠中脑内多巴胺转运蛋白（DAT）、胰岛素样生长因子1受体（IGF-1R）与脑源性神经营养因子（BDNF）mRNA水平的影响。结果雌激素处理可以增加小鼠黑质多巴胺神经元数量[E

组(119.7±2.9)个vs对照组(93.5±5.1)个

P=0.004

]

，并增加小鼠中脑内DAT的表达水平（P0.05），而对calbindin阳性神经元数量无影响[E

组(88.0±2.0)个vs对照组(86.2±1.5)个

]

。雌激素处理同时可以上调小鼠中脑内IGF-1R与BDNF mRNA水平（P0.05）。结论雌激素通过调节IGF-1R与BDNF的表达增加小鼠中脑内多巴胺神经元数量。

Abstract

Objective To determine the effect of estrogens (E

) on dopamine neurons in the substantia nigra (SN) of adult male mice and the underlying molecular mechanism. Methods Ten C57/BL6 mice were randomized to 2 groups

control group (with subcutaneous injection of 0.1 ml sesame oil for 10 d) and E

group (with subcutaneous injection of 2 μg/d 17-β estradiol solved in 0.1 ml sesame oil for 10 d). Immunohistochemistry was used to detect the positive neurons of tyrosine hydroxylase (TH) and calbindin. The expression levels of dopamine transporters

(DAT)

insulin-like growth factor 1 receptor (IGF-1Rm) and brain-derived neurotrophic factor (BDNF) were detected by RT-PCR. Results E

increased the number of dopamine neurons [(119.7±2.9) vs (93.5±5.1)

]

(P=0.004) and the mRNA levels of DAT（P0.05）

but had no effect on the number of calbindin-positive neurons [(88.0±2.0) vs (86.2±1.5)

]

（P=0.507）. E

increased the mRNA levels of IGF-1R and BDNF in the midbrain significantly（P0.05）. Conclusion E

can increase the number of dopamine neurons through upregulating the expression of IGF-1R and BDNF.

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