Silencing HIF-1α<sup>516</sup> inhibits invasive ability of HepG2 cells

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Silencing HIF-1α⁵¹⁶ inhibits invasive ability of HepG2 cells

更新时间：2026-01-09

- Silencing HIF-1α⁵¹⁶ inhibits invasive ability of HepG2 cells
- Journal of Army Medical University Vol. 33, Issue 2, Pages: 169-172(2011)
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  CLC： R394.3;R73-37;R735.7
- Published：2011
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邓雪松,王立.HIF-1α516对人肝癌HepG2细胞侵袭转移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(02):169-172.

　Deng Xuesong,Wang Li.Silencing HIF-1α516 inhibits invasive ability of HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(02):169-172.
邓雪松,王立.HIF-1α516对人肝癌HepG2细胞侵袭转移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(02):169-172. DOI：

　Deng Xuesong,Wang Li.Silencing HIF-1α516 inhibits invasive ability of HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(02):169-172. DOI：

摘要

目的观察HIF-1α

516

对人肝癌HepG2细胞侵袭转移能力的影响。方法人肝癌HepG2细胞分为空白组（同步培养，不做任何处理）、对照组（转染siRNA）和siRNA干扰组（转染HIF-1α

516

siRNA）。siRNA和HIF-1α

516

siRNA通过脂质体转染HepG2，转染18 h 后Western blot 检测HepG2细胞中HIF-1β核蛋白和全细胞蛋白的表达、ELISA法检测细胞培养上清中MMP-2、MMP-9的含量，Transwell小室观察HepG2的转移侵袭能力。结果 HIF-1α

516

siRNA能显著降低HepG2细胞HIF-1α

516

mRNA的表达（降低70.0%）（P0.01）；增强HepG2细胞的侵袭转移能力（增强71.10%）（P0.01）。HIF-1α

516

siRNA对全细胞HIF-1β蛋白的表达无影响，但能促进HIF-1β蛋白的核转移（增强64.1%）（P0.01）。HIF-1α

516

siRNA能明显升高HepG2细胞培养上清中MMP-2、MMP-9蛋白表达，分别升高37.72%、55.46% (P0.01)。结论 HIF-1α

516

能抑制HepG2的转移侵袭，其机制可能与抑制HIF-1β的核转移有关。

Abstract

Objective To explore the effect of HIF-1α

516

on invasive ability of HepG2 cells. Methods HepG2 cells were divide into blank group

control group and HIF-1α

516

siRNA groups

The blank group received no intervention

and the control group and HIF-1α

516

siRNA group were transfected by siRNA control and HIF-1α

516

siRNA

respectively with the aid of Liposome. In 18 h later

the effect of HIF-1α

516

siRNA on HepG2 cells invasive ability in vitro was measured by Boyden chamber invasive models. The expression of HIF-1α

516

mRNA were measured by real-time PCR. Expression of HIF-1β were detected by Western blot analysis

and the contents of MMP-2 and MMP-9 in the supernatant was measured by ELISA. Results In HIF-1α

516

siRNA group

the expression of HIF-1α

516

mRNA were decreased by 70.0%

and contents of MMP-2 and MMP-9 protein were increased by 37.72% and 55.46% (P0. 01)

respectively. The invasive ability of the HIF-1α

516

siRNA transfected cells was increased by 71.1% (P0.01). HIF-1α

516

siRNA had no effect on HIF-1β protein expression in HepG2 cells

but it increased the HIF-1β protein expression in HepG2 nucleus by 64.1%（P0.01）. Conclusion Silencing HIF-1α

516

suppresses the invasive ability of HepG2 cells. This may be concerned with that it could inhibit the nuclear transfer of HIF-1β.

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