Silencing △Np63 suppresses proliferation and improves apoptosis in transitional cell carcinoma of the bladder in vitro by upregulating p27<sup>kip1</sup> and p57<sup>kip2</sup>

张翾; 张家模; 吴小候; 何云锋

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Silencing △Np63 suppresses proliferation and improves apoptosis in transitional cell carcinoma of the bladder in vitro by upregulating p27^kip1 and p57^kip2

更新时间：2026-01-09

- Silencing △Np63 suppresses proliferation and improves apoptosis in transitional cell carcinoma of the bladder in vitro by upregulating p27^kip1 and p57^kip2
- Journal of Army Medical University Vol. 33, Issue 2, Pages: 141-144(2011)
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  CLC： R73-362;R730.23;R737.14
- Published：2011
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张翾,张家模,吴小候,何云锋.△Np63基因沉默对膀胱癌细胞增殖和凋亡的影响及分子机制[J].陆军军医大学学报(原第三军医大学学报),2011,33(02):141-144.

　Zhang Xuan,Zhang Jiamo,Wu Xiaohou,He Yunfeng.Silencing △Np63 suppresses proliferation and improves apoptosis in transitional cell carcinoma of the bladder in vitro by upregulating p27kip1 and p57kip2[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(02):141-144.
张翾,张家模,吴小候,何云锋.△Np63基因沉默对膀胱癌细胞增殖和凋亡的影响及分子机制[J].陆军军医大学学报(原第三军医大学学报),2011,33(02):141-144. DOI：

　Zhang Xuan,Zhang Jiamo,Wu Xiaohou,He Yunfeng.Silencing △Np63 suppresses proliferation and improves apoptosis in transitional cell carcinoma of the bladder in vitro by upregulating p27kip1 and p57kip2[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(02):141-144. DOI：

摘要

目的探讨癌基因△Np63对膀胱癌细胞增殖和凋亡的影响，并初步探讨其可能的分子机制。方法通过将△Np63特异性短发夹RNA(△Np63-shRNA)和非特异性短发夹RNA（Control-shRNA）转染膀胱移行细胞癌（transitional cell carcinoma of bladder

TCCB）5637细胞，用半定量逆转录-聚合酶链反应(RT-PCR)和Western blot分别检测△Np63、p27

kip1

、p57

kip2

mRNA和蛋白的表达水平，WST-1法检测细胞增殖活性，利用流式细胞术(FCM)检测细胞周期分布，TUNEL法检测细胞凋亡情况。结果特异性的△Np63-shRNA能够有效地沉默△Np63并上调p27

kip1

和p57

kip2

的基因和蛋白水平，转染△Np63-shRNA的5637细胞的增殖能力明显受到抑制（P0.05），且明显促进了细胞的凋亡(P0.05)。结论靶向△Np63基因的shRNA片段可以有效的抑制人膀胱癌5637细胞的增殖并促进其凋亡，其可能的机制是通过上调p27

kip1

和p57

kip2

的表达实现的。

Abstract

Objective To investigate the influence of △Np63 gene on the proliferation and apoptosis of transitional cell carcinoma of bladder (TCCB) 5637 cells

and explore its underlying molecular mechanism. Methods TCCB 5637 cells were transfected with △Np63-shRNA or control-shRNA expression vector. mRNA and protein levels of △Np63

p27

kip1

and p57

kip2

were measured by RT-PCR and Western bolt analysis

respectively. The cell proliferation was assessed by WST-1 assay

the change in cell cycle was evaluated by flow cytometry

and cell apoptosis was detected by TUNEL. Results The specific shRNA targeted to △Np63 gene downregulated the expression of △Np63 and upregulated the expressions of p27

kip1

and p57

kip2

at mRNA and protein levels. The proliferation of 5637 cells was markedly inhibited and the apoptosis of 5637 cells was increased after transfection eith △Np63-shRNA (P0.05). Conclusion △Np63-shRNA effectively inhibits the expression of △Np63

and suppresses cell growth and improves cell apoptosis by upregulating the expressions of p27

kip1

and p57

kip2

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Silencing △Np63 suppresses proliferation and improves apoptosis in transitional cell carcinoma of the bladder in vitro by upregulating p27kip1 and p57kip2

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Silencing △Np63 suppresses proliferation and improves apoptosis in transitional cell carcinoma of the bladder in vitro by upregulating p27^kip1 and p57^kip2