Trichostatin A enhances transfection efficiency of oncolytic adenovirus in vitro through up-regulating CAR in human esophageal cancer cell line EC9706

冯婷; 刘霞; 黄幼田; 杨红艳; 董子明

您当前的位置：

首页 >

文章列表页 >

Trichostatin A enhances transfection efficiency of oncolytic adenovirus in vitro through up-regulating CAR in human esophageal cancer cell line EC9706

更新时间：2026-01-09

- Trichostatin A enhances transfection efficiency of oncolytic adenovirus in vitro through up-regulating CAR in human esophageal cancer cell line EC9706
- Journal of Army Medical University Vol. 32, Issue 22, Pages: 2404-2408(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.2;R730.53;R735.1
- Published：2010
- 稿件说明：
移动端阅览
冯婷,刘霞,黄幼田,杨红艳,董子明.曲古菌素A上调食管癌CAR的表达及增强溶瘤腺病毒转染效率的体外研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(22):2404-2408.

　Feng Ting,Liu Xia,Huang Youtian,Yang Hongyan,Dong Ziming.Trichostatin A enhances transfection efficiency of oncolytic adenovirus in vitro through up-regulating CAR in human esophageal cancer cell line EC9706[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(22):2404-2408.
冯婷,刘霞,黄幼田,杨红艳,董子明.曲古菌素A上调食管癌CAR的表达及增强溶瘤腺病毒转染效率的体外研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(22):2404-2408. DOI：

　Feng Ting,Liu Xia,Huang Youtian,Yang Hongyan,Dong Ziming.Trichostatin A enhances transfection efficiency of oncolytic adenovirus in vitro through up-regulating CAR in human esophageal cancer cell line EC9706[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(22):2404-2408. DOI：

摘要

目的通过观察组氨酸去乙酰化酶（histone deacetylase，HDAC)抑制剂曲古菌素（trichostatin A

TSA）对食管癌细胞株EC9706膜表面柯萨奇-腺病毒受体（Coxsackie and adenovirus receptor，CAR）表达水平的影响，观察TSA能否通过上调食管癌EC9706 CAR表达而增强其对腺病毒基因转染效率。方法采用免疫细胞组织化学、RT-PCR、Western blot检测食管癌EC9706细胞CAR的表达情况，再用上述方法检测TSA作用EC9706细胞后，CAR mRNA和CAR蛋白表达的改变；同时采用流式细胞术测定病毒转染效率，MTT实验评价腺病毒携带的胸苷激酶（Ad-TK)的体外抗瘤效应。结果 0.5 μmol/L TSA处理EC9706细胞12、24、48 h后，CAR mRNA和蛋白表达量[分别为（0.44±0.01）、（0.37±0.02）

（0.66±0.04）、（0.58±0.01）

（0.77±0.05）、（0.70±0.03）]与未加TSA处理组[(0.27±0.02)、(0.22±0.03)]相比，CAR mRNA和蛋白表达水平显著提高（P0.05，P0.01），呈现出时间依赖关系。流式细胞仪分析AdGFP转染后GFP的表达发现，未加TSA处理细胞转染效率为(1.35±0.11)%，0.5 μmol/L TSA作用12、24 h 和48 h后，细胞转染效率分别为（2.58±0.17）%、（4.96±0.14）%、（6.36±0.15）%。MTT检测Ad-TK介导的体外杀伤作用结果显示，0.5 μmol/L TSA作用48 h比未加TSA增强8倍。结论 TSA通过上调CAR表达量从而增强腺病毒对食管癌细胞的腺病毒基因转染效率。

Abstract

Objective To explore the effect of histone deacetylase (HDAC) inhibitor

trichostatin A (TSA)

on the expression of Coxsackie and adenovirus receptor (CAR) in esophageal cancer cell line EC9706. Methods Immunocytochemistry

RT-PCR and Western blotting were used to detect the expression of CAR in EC9706 cells

and its mRNA and protein expressions after the treatment of 0.5 mol/L TSA for 12

24 and 48 h were also studied. Transfection efficiency of virus Ad-GFP in the EC9706 cells with or without 0.5 mol/L TSA treatment was determined by flow cytometry. Anti-tumor effect of adenovirus carrying thymidine kinase (Ad-TK) was evaluated by MTT assay. Results After 0.5 μmol/L TSA treatment for 12

24 and 48 h

CAR mRNA and protein expression were 0.44±0.01 and 0.37±0.02

0.66±0.04 and 0.58±0.01

and 0.77±0.05 and 0.70±0.03 respectively. Compared to those of control group (0.27±0.02 and 0.22±0.03)

the CAR mRNA and protein expressions were significantly increased in EC9706 cells after TSA treatment (P0.05

0.01)

and the expressions showed a time-dependent manner. Flow cytometry indicated that the transfection efficiency was (1.35±0.11)% in the EC9706 cells

but was (2.58±0.17)%

(4.96±0.14)%

and (6.36±0.15)% respectively in the cells after 0.5 μmol/L TSA treatment for 12

24 and 48 h. MTT results showed that the Ad-TK-mediated cytotoxicity in the cells with 0.5 μmol/L TSA treatment for 48 h was eight times higher than in the cells untreated. Conclusion TSA can increase gene transfer efficiency of adenovirus into cancer cells by upregulating CAR.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Concern over global outbreak of acute severe hepatitis of unknown etiology in children

Expression and mechanism of rat hyperplasia suppressor gene suppressing growth of rat C6 glioma cells

Construction and expression of recombinant adenovirus vector of erythropoietin in rat spiral ganglion neurons

Construction of recombinant adenovirus with siANGPTL4 gene and its inhibitive effect on MG63 cell proliferation

Role and mechanism of protein kinase D3 in promoting migration of non-small cell lung cancer A549 cells

Related Author

赵颂涛

毛青

沈冰

赵巍

方志豪

杨强

郭晖

蒋树财

Related Institution

No data

⁰