Objective   To study the inhibitory and biological effect of prS1 molecule conjugate carrying siHBX on expression of the HBX gene in HepG2-HBX cell line.    Methods    pres1 molecule conjugate and siHBX were chemically synthesized. Ability of the preS1 molecule conjugate to bind to HepG2-HBX and siRNA was tested. The siRNA was then transfected into the HepG2-HBX cell line by the preS1 molecule conjugate. Inhibiting effect of siHBX on expression of the HBX gene was detected by quantitative real-time RT-PCR and Western blotting

respectively.    Results   The HepG2-HBX cell line stably expressing the HBX gene was successfully constructed. The preS1 molecule conjugate could effectively bind to siHBX and inhibit expression of  HBX in HepG2-HBX cells. The inhibition rate of pres1 molecule conjugate on expression of HBX was significantly lower in negative control groups than in postive and experimental group   (11.94%±6.3% vs 85.24%±2.6% and 77.80%±6.1%

P0.05)     Conclusion    preS1 molecule conjugate carrying siHBX can be used as a novel siRNA carrier and plays a role in interference with RNA in liver cancer cells

thus providing a new treatment modality for HBV-related liver diseases.