Isolation and identification of ovarian cancer stem cells from ovarian cancer cell line A2780

谢伟; 龙海霞; 张恒; 向橦; 朱波; 谢荣凯

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Isolation and identification of ovarian cancer stem cells from ovarian cancer cell line A2780

更新时间：2026-01-09

- Isolation and identification of ovarian cancer stem cells from ovarian cancer cell line A2780
- Journal of Army Medical University Vol. 33, Issue 4, Pages: 356-359(2011)
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  CLC： R329.2;R73-354;R737.31
- Published：2011
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谢伟,龙海霞,张恒,向橦,朱波,谢荣凯.人卵巢癌细胞系A2780中肿瘤干细胞的分离及鉴定[J].陆军军医大学学报(原第三军医大学学报),2011,33(04):356-359.

　Xie Wei,Long Haixia,Zhang Heng,Xiang Tong,Zhu Bo,Xie Rongkai.Isolation and identification of ovarian cancer stem cells from ovarian cancer cell line A2780[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(04):356-359.
谢伟,龙海霞,张恒,向橦,朱波,谢荣凯.人卵巢癌细胞系A2780中肿瘤干细胞的分离及鉴定[J].陆军军医大学学报(原第三军医大学学报),2011,33(04):356-359. DOI：

　Xie Wei,Long Haixia,Zhang Heng,Xiang Tong,Zhu Bo,Xie Rongkai.Isolation and identification of ovarian cancer stem cells from ovarian cancer cell line A2780[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(04):356-359. DOI：

摘要

目的从人卵巢癌细胞系A2780中分离并鉴定卵巢癌干细胞。方法将A2780细胞置于含人重组表皮生长因子（epidermal growth factor

EGF）、人重组碱性成纤维生长因子（basic fibroblast growth factor

bFGF)、牛血清白蛋白和人胰岛素的无血清培养基中培养，采用连续传代诱导培养肿瘤干细胞，对每代分离出来的肿瘤干细胞进行流式检测，并对第4代肿瘤干细胞进行免疫荧光检测和逆向分化实验。结果从A2780细胞中成功分离出肿瘤干细胞，在上述无血清培养基中呈悬浮生长，具有很强的自我更新能力，流式检测显示随着传代诱导，CD133阳性率依次升高，免疫荧光进一步证实了CD133是卵巢癌干细胞的表面标记物，诱导分化实验说明其可逆向分化成为A2780肿瘤细胞。结论体外培养的卵巢癌细胞株A2780中存在卵巢癌干细胞，并能将其分离培养和诱导分化。

Abstract

Objective To isolate and identify ovarian cancer stem cells from ovarian cancer cell line A2780. Methods The A2780 cells were cultured in serum-free medium with EGF

bFGF

insulin and BSA to induce tumor stem cells by continuous passage. Flow cytometry was applied to identify the cancer stem cells in every passage. Immunofluorescence staining and reverse differentiation experiments were conducted on the fourth passage of cancer stem cells. Results The ovarian cancer stem cells were successfully isolated from ovarian cancer cell line A2780. They formed typical neurospheres in serum-free medium

and had strong self-renew capacity. The results of flow cy

tometry illustrated that CD133 positive rate was increased with the passages of cancer stem cells. Immunofluorescence staining further confirmed that CD133

cells were the surface marker of ovarian cancer stem cells

and they could differentiate into ovarian cancer cells in reverse differentiation experiments. Conclusion There were ovarian cancer stem cells in ovarian cancer cell line A2780

which can be isolated

cultured and differentiated.

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