Effect of static magnetic field at different strengths on Ca<sup>2+</sup> concentration in rat primarily cultured osteoblasts

王胜国; 周力; 陈扬熙; 王艳民; 任晓华

您当前的位置：

首页 >

文章列表页 >

Effect of static magnetic field at different strengths on Ca²⁺ concentration in rat primarily cultured osteoblasts

更新时间：2026-01-09

- Effect of static magnetic field at different strengths on Ca²⁺ concentration in rat primarily cultured osteoblasts
- Journal of Army Medical University Vol. 32, Issue 23, Pages: 2515-2518(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.71;R329.26;R339.5
- Published：2010
- 稿件说明：
移动端阅览
王胜国,周力,陈扬熙,王艳民,任晓华.不同强度静磁场对成骨细胞细胞内钙离子浓度的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(23):2515-2518.

　Wang Shengguo,Zhou Li,Chen Yangxi,Wang Yanmin,Ren Xiaohua.Effect of static magnetic field at different strengths on Ca2+ concentration in rat primarily cultured osteoblasts[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(23):2515-2518.
王胜国,周力,陈扬熙,王艳民,任晓华.不同强度静磁场对成骨细胞细胞内钙离子浓度的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(23):2515-2518. DOI：

　Wang Shengguo,Zhou Li,Chen Yangxi,Wang Yanmin,Ren Xiaohua.Effect of static magnetic field at different strengths on Ca2+ concentration in rat primarily cultured osteoblasts[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(23):2515-2518. DOI：

摘要

目的对成骨细胞静磁场加载过程中细胞内的钙离子浓度进行动态定量检测，探讨成骨细胞静磁场加载过程中细胞内钙离子浓度的变化规律。方法采用静磁场加载装置，对体外培养的成骨细胞分别在8、50 mT和160 mT的磁场强度下给予24、48 h和72 h的静磁场加载；0 mT作为对照组，不进行静磁场加载，只受地磁场的影响。应用邻甲酚酞络合铜（OCPC）微板法测量加载后细胞内钙离子浓度。结果原代培养的细胞经鉴定具有成骨细胞的生物学特征，为成骨细胞。与0 mT组相比，8、50 mT和160 mT的静磁场加载后24、48、72 h成骨细胞内的钙离子浓度均降低(P0.05)。与0 mT组比较，8 mT组在24、48、72 h细胞内钙离子浓度降低率分别为27.48%、24.41%和10.46%，50 mT组降低率分别为19.03%、18.04%和15.54%，160 mT组降低率分别为8.58%、13.10%和19.03%。结论一定强度的静磁场加载能够降低成骨细胞内的钙离子浓度。

Abstract

Objective To investigate the Ca

concentration in osteoblasts and to study its changing rule during static magnetic field loading. Methods Osteoblasts primarily cultured form 1-day rat skull were cultured under 0

50 and 160 mT of static magnetic field for 24

48 and 72 h

respectively. The cells cultured under 0 mT used as control. Osteoblasts were identified with alkaline phosphatase (ALP) and Von Kossa stainings. Ca

concentrations were measured with the OCPC micro-plating method and analyzed by variance. Results The culuted cells showed

the biological features of osteoblasts and positive to ALP and Von Kossa stainings. Compared with the cells under 0 mT field

the Ca

concentration of 8 mT group was decreased by 27.48%

24.41% and 10.46% respectively at 24

48 and 72 h

that of 50 mT group was by 19.03%

18.04% and 15.54%

and that of 160 mT group was by 8.58%

13.10% and 19.03% respectively for different time points. Significant reduce was found in the Ca

concentration after static magnetic field exposure compared with that of normal control (P0.05). Conclusion The static magnetic field at a certain strength can decrease the Ca

concentration in osteoblasts.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of static magnetic field on proliferation and surface ultra-structure of osteoblasts in vitro

Effect of genistein on proliferation and differentiation of osteoblasts isolated and cultured from ovariectomized rats

Bone morphogenetic protein receptor 1A-mediated signaling pathway regulates osteoblast terminal differentiation in vivo

Study on biological characteristics of subchondral bone osteoblasts in osteoarthritis patients

Osteoblasts and vascular endothelial cells combined with allogeneic morselized bone graft for treating rat osteonecrosis of the femoral head

Related Author

任晓华

陈扬熙

王艳民

周力

王胜国

高玉海

李娟

张涛

Related Institution

No data

⁰

Effect of static magnetic field at different strengths on Ca2+ concentration in rat primarily cultured osteoblasts

DOI：

摘要

Abstract

关键词

Keywords

references

Effect of static magnetic field at different strengths on Ca²⁺ concentration in rat primarily cultured osteoblasts