Ultrasound-activated hematoporphyrin promotes apoptosis of cardiomyocytes of neonatal rats

王均生; 刘地川; 黄晶; 吴泓权; 童欣

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Ultrasound-activated hematoporphyrin promotes apoptosis of cardiomyocytes of neonatal rats

更新时间：2026-01-09

- Ultrasound-activated hematoporphyrin promotes apoptosis of cardiomyocytes of neonatal rats
- Journal of Army Medical University Vol. 32, Issue 24, Pages: 2604-2607(2010)
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  CLC： R454.3;R363.27;R322.1
- Published：2010
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王均生,刘地川,黄晶,吴泓权,童欣.超声激活血卟啉促进新生大鼠心肌细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(24):2604-2607.

　Wang Junsheng,Liu Dichuan,Huang Jing,Wu Hongquan,Tong Xin.Ultrasound-activated hematoporphyrin promotes apoptosis of cardiomyocytes of neonatal rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(24):2604-2607.
王均生,刘地川,黄晶,吴泓权,童欣.超声激活血卟啉促进新生大鼠心肌细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(24):2604-2607. DOI：

　Wang Junsheng,Liu Dichuan,Huang Jing,Wu Hongquan,Tong Xin.Ultrasound-activated hematoporphyrin promotes apoptosis of cardiomyocytes of neonatal rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(24):2604-2607. DOI：

摘要

目的观察超声激活血卟啉对新生SD大鼠心肌细胞的凋亡效应，并初步探讨其相关机制。方法采用差速贴壁法培养原代新生SD大鼠心肌细胞，将其分为4组：超声（频率300 kHz

声强1.5 W/cm

2，

辐照60 s）+血卟啉组（浓度20 μg/ml），超声组（频率300 kHz

声强1.5 W/cm

，辐照60 s），血卟啉组（浓度20 μg/ml）和对照组。采用MTT法观察各处理因素对细胞的生长抑制情况；流式细胞仪膜连蛋白 V-FITC/PI双染法检测细胞凋亡状况；免疫荧光细胞化学法鉴定细胞及检测Fas/FasL蛋白表达情况。结果 MTT法测得24 h抑制率超声+血卟啉组显著高于其他各组（P0.05）。膜连蛋白 V-FITC /PI双染法检测超声+血卟啉组细胞早期凋亡率明显高于其他各组（P0.05）。与其他各组相比，免疫荧光细胞化学法测定超声+血卟啉组Fas/FasL蛋白表达光密度值有明显增高（P0.05）。结论超声激活血卟啉对心肌细胞有明显的凋亡效应，其可能与Fas/FasL蛋白表达增高有关。

Abstract

Objective To investigate the effect of ultrasound-activated hematoporphyrin on apoptosis of cardiomyocytes from neonatal SD rats and to explore the underlying mechanism. Methods The primary cultured cardiomyocytes from neonatal rats were randomly divided into 4 groups

ultrasound (frequency 300 kHz

sound intensity 1.5 W/cm

irradiation 60 s) plus hematoporphyrin (concentration 20 μg/ml)

ultrasound (frequency 300 kHz

sound intensity 1.5 W/cm

irradiation 60 s)

hematoporphyrin (concentration 20 μg/ml) and control group. The cell growth inhibitory effect of each

group was observed by MTT assay. Apoptosis was measured by flow cytometry with Annexin V-FITC/PI staining. The expressions of Fas and FasL were identified by immunocytochemistry. Results The growth of the cultured cardiomyocytes of neonatal rats was significantly inhibited by hematoporphyrin combined with ultrasound at 24 h after treatment (P0.05). The apoptotic rate of ultrasound plus hematoporphyrin group at early term was obviously higher than those of other groups by Annexin V-FITC/PI assay (P0.05). Compared wtih other groups

hematoporphyrin combined with ultrasound apparently increased the level of Fas/FasL protein (P0.05). Conclusion Ultrasound-activated hematoporphyrin enhances the apoptosis of cardiomyocytes of neonatal rats

which is possibly associated with higher expression of Fas/FasL protein.

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