Population, proliferative ability and suppressive activity of CD4<sup>+</sup>CD25<sup>+</sup> regulatory T cells in MRL/lpr mice

冯林; 刁庆春; 郑峻松; 贺娟; 吴军

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Population, proliferative ability and suppressive activity of CD4⁺CD25⁺ regulatory T cells in MRL/lpr mice

更新时间：2026-01-09

- Population, proliferative ability and suppressive activity of CD4⁺CD25⁺ regulatory T cells in MRL/lpr mice
- Journal of Army Medical University Vol. 33, Issue 4, Pages: 353-355(2011)
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  CLC： R-332;R392.12;R593.241
- Published：2011
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冯林,刁庆春,郑峻松,贺娟,吴军.MRL/lpr小鼠中CD4+CD25+调节性T细胞含量、增殖能力及抑制能力的研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(04):353-355.

　Feng Lin,Diao Qingchun,Zheng Junsong,He Juan,Wu Jun.Population, proliferative ability and suppressive activity of CD4+CD25+ regulatory T cells in MRL/lpr mice[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(04):353-355.
冯林,刁庆春,郑峻松,贺娟,吴军.MRL/lpr小鼠中CD4+CD25+调节性T细胞含量、增殖能力及抑制能力的研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(04):353-355. DOI：

　Feng Lin,Diao Qingchun,Zheng Junsong,He Juan,Wu Jun.Population, proliferative ability and suppressive activity of CD4+CD25+ regulatory T cells in MRL/lpr mice[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(04):353-355. DOI：

摘要

目的探讨MRL/lpr小鼠中CD4

CD25

调节性T细胞（Treg细胞）的含量、增殖能力及其对效应性T淋巴细胞（Teff细胞）的抑制能力及意义。方法流式细胞检测法检测8只MRL/lpr小鼠及8只BALB/c小鼠脾脏中Treg细胞的水平；磁珠分选法分选出脾脏中的Treg细胞与Teff细胞，离体培养2周后，分别测量Treg细胞数量，并比较差异；用Treg细胞与Teff细胞组成共培养体系，5 d后检测Teff细胞的水平，计算Treg细胞对Teff细胞的抑制率并比较差异。结果 MRL/lpr小鼠脾脏中Treg细胞的总含量与BALB/c小鼠无统计学差异[（4.88±0.55）% vs（5.03±0.34）%，P0.05

]

；离体培养2周后MRL/lpr小鼠的Treg细胞数量显著低于BALB/c小鼠[(6.61±0.92)×10

vs (13.25±1.91)×10

，P0.05

]

；共培养5 d后MRL/lpr小鼠Treg细胞对Teff细胞的抑制率显著低于BALB/c小鼠Treg细胞（P0.05）。结论 MRL/lpr小鼠体内Treg细胞增殖能力、抑制能力的下降可能导致体内免疫平衡的破坏、自身抗原-抗体反应，参与MRL/lpr小鼠的系统性红斑狼疮的发病。

Abstract

Objective To investigate the percentage

reproductive ability of CD4

CD25

regulatory T cells (Tregs) in MRL/lpr mice

and their suppressive activity to effective T cells (Teffs). Methods Flow cytometry was used to determine cell populations of Tregs in the spleen of 8 MRL/lpr mice and 8 BALB/c mice respectively. Magnetic beads were employed to separate Tregs and Teffs from the sple

nic cells of the 2 kinds of mice. Then the Tregs were cultured in vitro for 2 weeks and cell populations were determined. The Tregs were cultured with Teffs for 5 d and then the level of Teffs was detected. The suppresive rate of Tregs to Teffs was calculated. Results No significant difference was detected in cell populations of Tregs between 2 kinds of mice [(4.88±0.55)% and (5.03±0.34)%

respectively; P0.05

]

. After cultivation in vitro for 2 weeks

cell populations of Tregs were significantly lower in MRL/lpr mice than that in BALB/c mice [(6.61±0.92)×10

and (13.25±1.91)×10

respectively; P0.05

]

. After co-cultivation in vitro for 5 d

the suppressive rate of Tregs to Teffs was significantly lower in MRL/lpr mice than that in BALB/c mice (P0.05). Conclusion The Tregs in MRL/lpr mice have decreased reproductive ability and suppressive activity

which may lead to impairment of immune balance and induction of auto-antibody

and thus the illness of systemic lupus erythematosus .

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Population, proliferative ability and suppressive activity of CD4+CD25+ regulatory T cells in MRL/lpr mice

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Population, proliferative ability and suppressive activity of CD4⁺CD25⁺ regulatory T cells in MRL/lpr mice