Mangiferin up-regulates expression of membrane protein MRP3 and nuclear receptors PXR and CPF in HepG2 cells

张艳梅; 柴进; 阳勇; 张婷; 罗维早; 钟国跃; 陈文生

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Mangiferin up-regulates expression of membrane protein MRP3 and nuclear receptors PXR and CPF in HepG2 cells

更新时间：2026-01-09

- Mangiferin up-regulates expression of membrane protein MRP3 and nuclear receptors PXR and CPF in HepG2 cells
- Journal of Army Medical University Vol. 33, Issue 3, Pages: 246-249(2011)
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  CLC： R394.2;R730.23;R735.7
- Published：2011
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张艳梅,柴进,阳勇,张婷,罗维早,钟国跃,陈文生.芒果苷上调HepG2细胞膜蛋白MRP3和核受体PXR、CPF表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(03):246-249.

　Zhang Yanmei,Chai Jin,Yang Yong,Zhang Ting,Luo Weizhao,Zhong Guoyue,Chen Wensheng.Mangiferin up-regulates expression of membrane protein MRP3 and nuclear receptors PXR and CPF in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(03):246-249.
张艳梅,柴进,阳勇,张婷,罗维早,钟国跃,陈文生.芒果苷上调HepG2细胞膜蛋白MRP3和核受体PXR、CPF表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(03):246-249. DOI：

　Zhang Yanmei,Chai Jin,Yang Yong,Zhang Ting,Luo Weizhao,Zhong Guoyue,Chen Wensheng.Mangiferin up-regulates expression of membrane protein MRP3 and nuclear receptors PXR and CPF in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(03):246-249. DOI：

摘要

目的体外HepG2细胞培养观察芒果苷（mangiferin）对多耐药相关蛋白3（multidrug resistance-associate protein 3

MRP3）和核受体孕烷X受体（pregnane X receptor，PXR）、CYP7A启动子结合因子（CYP7A promoter-binding factor，CPF）表达的影响。方法用芒果苷刺激HepG2细胞72 h后，分别抽提细胞RNA、膜蛋白及核蛋白，采用半定量RT-PCR和蛋白免疫印迹检测膜转运蛋白MRP3和核受体PXR、CPF在转录与蛋白水平的表达变化。熊脱氧胆酸（ursodeoxycholic acid

UDCA）处理的HepG2细胞作为阳性对照、DMSO处理细胞为阴性对照。结果芒果苷可显著刺激HepG2细胞膜转运蛋白MRP3的mRNA（比阴性对照组高3.0倍，P0.05）和蛋白(比阴性对照组高3.3倍，P0.05)表达，其作用强于UDCA。芒果苷也可明显上调核受体PXR [mRNA水平增高1.7倍(P0.05)，蛋白水平增高3.7倍(P0.01）]、CPF[mRNA水平增高2.1倍(P0.05)，蛋白水平增高4.9倍(P0.05）]的表达。结论芒果苷刺激肝癌细胞HepG2细胞膜转运蛋白MRP3的表达上调可能与核受体PXR、CPF途径相关。

Abstract

Objective To observe the effect of mangiferin on expressions of multidrug resistance-associated protein 3 (MRP3)

nuclear pregnane X receptor (PXR)

and CYP7A promoter-binding factor (CPF) in HepG2 cells. Methods Total RNA and membrane or nuclear protein were extracted from HepG2 cells 72 h after treated with mangiferin. Expressions of PXR and CPF at transcription and protein levels were detected by semi-quantitative RT PCR and Western blotting

respectively. HepG2 cells treated with ursodeoxycholic acid (UDCA) and DMSO served as positive and negative controls

respectively. Results Mangiferin could significantly up-regulate the mRNA and protein expression of MRP3 in HepG2 cells. The mRNA and protein expression levels of MRP3 were 3-fold and 3.3-fold higher

respectively

in positive controls than in negative controls (P0.05). The effect of mangiferin was stronger than that of UDCA on the expression of MRP3 (P0.05). Mangiferin up-regulated the expression levels of PXR and CPF (1.7-fold)

mRNA of MRP3 (3.7-fold)

CPF (21-fold)

and protein of MRP3 (4.9-fold)

respectively

in HepG2 cells (P0.05). Conclusion Mangiferin can up-regulate the expression of MRP3 in HepG2 cells

which may be related with PXR and PXR.

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