Resveratrol alleviates cerebral ischemia-reperfusion injury in rats

任俊伟; 杨琴; 范层层; 陈娜

您当前的位置：

首页 >

文章列表页 >

Resveratrol alleviates cerebral ischemia-reperfusion injury in rats

更新时间：2026-01-09

- Resveratrol alleviates cerebral ischemia-reperfusion injury in rats
- Journal of Army Medical University Vol. 32, Issue 24, Pages: 2586-2590(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R282.71;R743.31;R965
- Published：2010
- 稿件说明：
移动端阅览
任俊伟,杨琴,范层层,陈娜.白藜芦醇减轻脑缺血再灌注损伤及其机制[J].陆军军医大学学报(原第三军医大学学报),2010,32(24):2586-2590.

　Ren Junwei,Yang Qin,Fan Cengceng,Chen Na.Resveratrol alleviates cerebral ischemia-reperfusion injury in rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(24):2586-2590.
任俊伟,杨琴,范层层,陈娜.白藜芦醇减轻脑缺血再灌注损伤及其机制[J].陆军军医大学学报(原第三军医大学学报),2010,32(24):2586-2590. DOI：

　Ren Junwei,Yang Qin,Fan Cengceng,Chen Na.Resveratrol alleviates cerebral ischemia-reperfusion injury in rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(24):2586-2590. DOI：

摘要

目的研究白藜芦醇（Res）对脑缺血再灌注损伤的保护作用及可能的机制。方法线栓法制作大鼠大脑中动脉栓塞(middle cerebral artery occlusion

MCAO)模型，缺血2 h再灌注。将76只大鼠分成假手术组(S组)、缺血再灌注组(I/R组)、Res低剂量组（15 mg/kg，I/R+R1组)和Res高剂量组（30 mg/kg，I/R+R2组)，每组19只。再灌注24 h，采用TTC法测量脑梗死体积，化学比色法测定血清及脑组织中MDA含量，RT-PCR及Western blot检测Nrf2、HO-1基因和蛋白表达，HE染色观察脑组织的病理改变。结果与I/R组比较，Res能明显缩小脑梗死体积[I/R组(42.67±2.51)% vs I/R+R1组(30.53±1.45)%、I/R+R2组（20.27±2.11）%，P0.01]，降低血清中MDA的含量[I/R组(9.29±0.42) nmol/ml vs I/R+R1组(4.03±0.08) nmol/ml、I/R+R2组（2.68±0.27）nmol/ml，P0.01]，及脑组织中MAD含量[I/R组（1.40±0.02）nmol/mg vs IR+R1组（1.19±0.07）nmol/mg、IR+R2组（0.85±0.03）nmol/mg，P0.01]。显著上调Nrf2、HO-1基因和蛋白的表达(P0.05)，改善脑组织的病理变化。结论 Res对局灶性脑缺血再灌注损伤有良好的保护作用，其机制可能是通过激活Nrf2/ARE信号通路进而增强抗氧化蛋白HO-1表达实现的。

Abstract

Objective To investigate the protective effect of resveratrol (Res) on cerebral ischemia and reperfusion (I/R) injury and its possible mechanism. Methods A total of 76 SD rats were randomized into 4 groups

that is

sham-operation group

I/R group

low dose of Res (15 mg/kg

I/R﹢R1 group) and high dose of Res (30 mg/kg

I/R﹢R2 group). Res at corresponding dose was injected to the rats peritoneally once per day for 7 d. And then middle cerebral artery occlusion (MCAO) model in rats was established by intraluminal thread block for 2 h and reperfusion thereafter. After 24 hours’ reperfusion

the content of MDA in the serum and brain tissue was determined with chemical colorimetry. 2

5-triphenyltetrazolium chloride (TTC) staining was used to measure infarct area of brain tissue

and RT-PCR and Western blot analysis were employed the expressions of Nrf2 and HO-1 at mRNA and protein levels. Pathological changes of brain tissue was observed with HE staining. Results Res reduced the infarct areas by (30.53±1.45)% in I/R+R1 group and (20.27±2.11)% in I/R+R2 group when compared with I/R group [(42.67±2.51)%

P0.01]

decreased the contents of MDA in the serum (4.03±0.08 and 2.68±0.27 vs 9.29±0.42 nmol/ml

P0.01) and brain tissue (1.19±0.07 and 0.85±0.03 vs 1.40±0.02 nmol/mg

P0.01)

significantly increased the protein and mRNA expression of both Nrf2 and HO-1 (P0.05)，and improved the pathological changes in brain tissue. Conclusion Res exerts protective effect on cerebral I/R injury in rats. The mechanism may imply the activation of Nrf2-ARE signaling pathway and thus enhance expression of antioxidant HO-1.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect and mechanism of dimethyl fumarate on septic cardiomyopathy in mice

Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB in vitro

Topical administration of cinnamic aldehyde accelerates wound healing in diabetic mice by activation of Nrf2 pathway

Protective effects of agmatine on oxidative stress induced by LPS in RAW264.7 cells

Role of SIRT1/UCP2 signaling pathway in resveratrol-induced inhibition of oxidative injury in vascular endothelial cells

Related Author

闵卫翔

陈学均

马德祥

刘宇

刘书婷

史梦曦

周曦

易龙

Related Institution

Department of Human Anatomy， Anhui Medical University

Laboratory of Stem Cell and Tissue Engineering， Chongqing Medical University

Institute of Field Surgery， Daping Hospital， Army Medical University

Department of Geriatrics and Special Service Medicine， First Affiliated Hospital（Southwest Hospital）， Army Medical University （Third Military Medical University）

达州市中西医结合医院药学科

⁰