Effect of pinaverium bromide on expression of aquaporin 3 in colon tissue of rats  with diarrhea-predominant irritable bowel syndrome

刘慧慧; 王艳杰; 刘旭东; 关洪全; 柴纪严; 赵金茹; 王德山

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Effect of pinaverium bromide on expression of aquaporin 3 in colon tissue of rats with diarrhea-predominant irritable bowel syndrome

更新时间：2026-01-09

- Effect of pinaverium bromide on expression of aquaporin 3 in colon tissue of rats with diarrhea-predominant irritable bowel syndrome
- Journal of Army Medical University Vol. 33, Issue 3, Pages: 233-236(2011)
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  CLC： R394.2;R574.4;R975.3
- Published：2011
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刘慧慧,王艳杰,刘旭东,关洪全,柴纪严,赵金茹,王德山.匹维溴胺对腹泻型肠易激综合征模型大鼠结肠组织AQP3表达影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(03):233-236.

　Liu Huihui,Wang Yanjie,Liu Xudong,Guan Hongquan,Chai Jiyan,Zhao Jinru,Wang Deshan.Effect of pinaverium bromide on expression of aquaporin 3 in colon tissue of rats with diarrhea-predominant irritable bowel syndrome[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(03):233-236.
刘慧慧,王艳杰,刘旭东,关洪全,柴纪严,赵金茹,王德山.匹维溴胺对腹泻型肠易激综合征模型大鼠结肠组织AQP3表达影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(03):233-236. DOI：

　Liu Huihui,Wang Yanjie,Liu Xudong,Guan Hongquan,Chai Jiyan,Zhao Jinru,Wang Deshan.Effect of pinaverium bromide on expression of aquaporin 3 in colon tissue of rats with diarrhea-predominant irritable bowel syndrome[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(03):233-236. DOI：

摘要

目的观察匹维溴胺对腹泻型肠易激综合征（D-IBS）模型大鼠血清以及结肠组织血管活性肠肽（vasoactive intestinal peptide，VIP）含量、结肠组织水通道蛋白3（aquaporin 3

AQP3）表达的影响，探讨其治疗D-IBS的作用机制。方法采用慢性应激与束缚相结合方法建立D-IBS大鼠模型。采用ELISA法检测VIP含量；采用Real time-PCR法和SABC免疫组化法检测AQP3的表达。结果在血清和结肠组织中VIP的含量，与对照组[(3.092±0.613)ng/ml

(3.811±0.275)ng/ml]比较，模型组[(10.460±1.666)ng/ml

(4.495±0.341) ng/ml]与匹维溴胺组[(5.639±1.163)ng/ml

(4.063±0.534)ng/ml]均升高（P0.05），尤以模型组升高的更为明显（P0.01）；两组AQP3的表达均下调（P0.01），尤以模型组AQP3蛋白表达下调显著。结论匹维溴胺治疗D-IBS的作用机制之一，可能与抑制结肠组织中VIP分泌和上调AQP3表达有关。

Abstract

Objective To study the serum vasoactive intestinal peptide (VIP) level and the effect of pinaverium bromide (PB) on expression of aquaporin 3 (AQP3) in colon tissue and diarrhea-predominant irritable bowel syndrome (D-IBS) model of rats. Methods A D-IBS rat model was established with chronic stress plus binding limbs. Level of VIP was measured by ELISA and expression of AQP3 was detected by real-time PCR and SABC

respectively. Results The VIP level in serum and colon tissue was higher in model group (10.46±1.666 and 4.495±0.341 ng/ml) and PB group (5.639±1.163 and 4.063±0.534 ng/ml) than in control group (3.092±0.613 and 3.811±0.275 ng/ml) (P0. 05)

which was particularly high in model group. The expression of AQP3 in colon tissue was remarkably decreased in model group and PB group

especially in model group (P0. 01). Conclusion PB improves D-IBS by inhibiting the secretion of VIP and up-regulating the AQP3 expression in colon tissue.

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