microRNA-133b inhibits growth of subcutaneous implantation tumor of human colorectal cancer in nude mice

胡桂; 陈道瑾; 李小荣; 杨开焰

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microRNA-133b inhibits growth of subcutaneous implantation tumor of human colorectal cancer in nude mice

更新时间：2026-01-09

- microRNA-133b inhibits growth of subcutaneous implantation tumor of human colorectal cancer in nude mice
- Journal of Army Medical University Vol. 32, Issue 16, Pages: 1758-1761(2010)
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  CLC： R735.35;R73-352;R73-362
- Published：2010
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胡桂,陈道瑾,李小荣,杨开焰.microRNA-133b抑制人结直肠癌裸鼠皮下种植瘤生长[J].陆军军医大学学报(原第三军医大学学报),2010,32(16):1758-1761.

　Hu Gui,Chen Daojin,Li Xiaorong,Yang Kaiyan.microRNA-133b inhibits growth of subcutaneous implantation tumor of human colorectal cancer in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(16):1758-1761.
胡桂,陈道瑾,李小荣,杨开焰.microRNA-133b抑制人结直肠癌裸鼠皮下种植瘤生长[J].陆军军医大学学报(原第三军医大学学报),2010,32(16):1758-1761. DOI：

　Hu Gui,Chen Daojin,Li Xiaorong,Yang Kaiyan.microRNA-133b inhibits growth of subcutaneous implantation tumor of human colorectal cancer in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(16):1758-1761. DOI：

摘要

目的探讨microRNA133b(miR133b)对人结直肠癌裸鼠皮下种植瘤生长的抑制作用，体内实验进一步验证miR-133b是一种新的抑癌基因。方法 30只裸鼠按随机数字表法分为接种人结直肠癌细胞SW-620细胞组、接种SW-620-HK细胞组及接种SW-620-133b细胞组，每组10只，建立裸鼠皮下种植瘤模型，记录并比较各组种植瘤生长情况，TUNEL法检测种植瘤细胞凋亡，荧光实时定量PCR检测各组种植瘤中miR-133b表达，Western blot检测c-met表达。 结果各组裸鼠皮下接种肿瘤细胞5 d后均成功致瘤，成瘤率100%；SW-620-133b组种植瘤生长速度显著低于SW-620与SW-620-HK组(P0.05)；终点时间第30天时，SW-620组裸鼠皮下种植瘤的体积为（3.56±0.79）cm

，SW-620-HK组为（3.24±0.68）cm

，显著大于SW-620-133b组肿瘤体积（0.85±0.14）cm

(P0.05)；SW-620-133b组种植瘤质量为(1.15±0.16)g，明显低于SW-620组(4.02±0.64)g及SW-620-HK组（3.81±0.61）g(P0.05)；SW-620-133b组肿瘤细胞凋亡率（30.28±3.47）%显著高于对照组SW-620-HK（7.26±0.84）% 及空白组SW-620（7.96±0.92）%(P0.05)；SW-620-133b组种植瘤中c-met表达量（0.19±0.02）较SW-620组（0.52±0.04）及SW-620-HK组（0.51±0.04）显著下降(P0.05)。结论外源性高表达miR-133b可显著抑制人结直肠癌裸鼠皮下种植瘤的生长。

Abstract

Objective To explore the inhibitive effects of microRNA - 133b (miR - 133b) on the growth of implanted subcutaneous tumors in human colorectal tumor - bearing nude mice

and to investigate whether miR - 133b is a new tumor suppressor

in vivo

. Methods Thirty nude mice

were randomized into 3 groups

SW - 620 group

SW - 620 - HK group and SW - 620 - 133b group. The animal model of subcutaneous implantation tumor in nude mice was established by corresponding injecting 5 × 10

SW - 620 cells

SW - 620 - HK (transfected with nonsense sequence) and SW - 620 - 133b (transfected with miR - 133b) subcutaneously

and the model was established when the tumor was 3 to 4 mm in diameter. The tumor growth in each group was recorded and compared. The mice was killed in 30 d. The cell apoptosis of implantation tumor was tested by TUNEL assay. The expression of miR - 133b and c - met in each group were examined by real - time PCR and Western blotting

respectively. Results The model animals of human colorectal tumor - bearing mice were successfully established

with a tumor - formation rate of 100%. The growth curves of planted tumor showed that the tumor growth rate of SW - 620 - 133b group was much slower than those of SW - 620 and SW - 620 - HK groups (

0 . 05). At the end point time

the average tumor volume of SW - 620 - 133b group was smaller than those of SW - 620 and SW - 620 - HK groups [(0.85 ± 0.14) cm

(3.56 ± 0.79) cm

and (3.24 ± 0.68) cm

0 . 05

]

. The average tumor weight of SW - 620 - 133b group was lighter than those of SW - 620 and SW - 620 - HK groups [(1.15 ± 0.16) g

(4.02 ± 0.64) and (3.81 ± 0.61) g

0 . 05

]

. The cell apoptosis of SW - 620 - 133b group was significantly higher than those of SW - 620 and SW - 620 - HK groups [(30.28 ± 3.47)%

(7.96 ± 0.92)% and (7 . 26 ± 0 . 84)%

0 . 05

]

. The expression of c - met in SW - 620 - 133b group was significantly deceased compared with those of SW - 620 and SW - 620 - HK groups [(0.19 ± 0.02)

(0.52 ± 0.04) and (0.51 ± 0.04)

0 . 05

]

. Conclusion The ectopic expression of miR - 133b can effectively inhibit the growth of tumor in human colorectal tumor - bearing nude mice.

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