Stress due to mother and infant isolation enhances excitability of pyramidal neurons in hippocampal CA1 of infantile rats

周浩; 刘烨; 梁锦平; 陈恒胜; 邵建玲

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Stress due to mother and infant isolation enhances excitability of pyramidal neurons in hippocampal CA1 of infantile rats

更新时间：2026-01-09

- Stress due to mother and infant isolation enhances excitability of pyramidal neurons in hippocampal CA1 of infantile rats
- Journal of Army Medical University Vol. 32, Issue 13, Pages: 1442-1445(2010)
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  CLC： R338.26;R338.8;R363.271
- Published：2010
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周浩,刘烨,梁锦平,陈恒胜,邵建玲.母子隔离应激增加幼鼠海马CA1区锥体神经元的兴奋性[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1442-1445.

　Zhou Hao,Liu Ye,Liang Jinping,Chen Hengsheng,Shao Jianling.Stress due to mother and infant isolation enhances excitability of pyramidal neurons in hippocampal CA1 of infantile rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1442-1445.
周浩,刘烨,梁锦平,陈恒胜,邵建玲.母子隔离应激增加幼鼠海马CA1区锥体神经元的兴奋性[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1442-1445. DOI：

　Zhou Hao,Liu Ye,Liang Jinping,Chen Hengsheng,Shao Jianling.Stress due to mother and infant isolation enhances excitability of pyramidal neurons in hippocampal CA1 of infantile rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1442-1445. DOI：

摘要

目的初步探讨母子隔离应激对幼鼠海马CA1区锥体神经元自发性放电特征和海马GABA

受体α

亚单位表达的影响及其与癫痫易感性的关系。方法 54只新生的SD大鼠幼鼠用于实验，雌雄不拘，随机分成3组（n=18），正常组(正常喂养，每天不用隔离)、隔离15 min组、隔离3 h组。在生后16 d，3组幼鼠分别行氯化锂匹罗卡品诱导的惊厥实验，全细胞膜片钳检测海马CA1区锥体神经元自发性放电特征，灌注取脑免疫组化检测海马GABA

受体α

亚单位表达情况。结果隔离3 h组体质量（24.60±1.89）g与正常组体质量（29.44±2.03）g比较明显减轻（P0.05）、幼鼠诱发惊厥的潜伏期隔离3 h组（11.50±2.10）min短于正常组(15.32±1.34)min（P0.05）、海马CA1区锥体神经元自发性放电频率隔离3h组（3.64±2.84）Hz要明显快于正常组（1.15±0.69）Hz（P0.01）、海马CA1区GABA

受体α

亚单位累计化学光密度，隔离3 h组（161.10±5.70）少于正常组（175.40±7.20）（P0.01）。隔离15 min组幼鼠体质量（29.34±1.85）g、诱发惊厥的潜伏期（16.47±1.73）min、海马CA1锥体神经元自发性放电频率（1.36±0.83）Hz、海马CA1区GABA

受体α

亚单位累计化学光密度（171.50±6.80），在以上几方面隔离15 min组和正常组比较均无统计学差异（P0.05）。结论早期母子隔离3 h的应激对幼鼠生长发育、癫痫易感性产生不良影响，海马CA1锥体神经元兴奋性增加、GABA

受体α

亚单位表达减少可能作为其潜在的病理机制之一。

Abstract

Objective To study the effect of stress due to mother and infant isolation on spontaneous discharge of pyramidal neurons in hippocampal CA1 and expression of hippocampal GABA

receptor α

in infantile rats

and its correlation with susceptibility to epilepsy. Methods Infantile rats were randomly divided into normal group

15-min isolation group

and 3-h isolation group. Sixteen days after birth

convulsion was induced in rats with lithium chloridepilocapine. Characteristics of spontaneous discharge of pyramidal neurons in hippocampal CA1 were detected with wholecell patch clamps. Expression of hippocampal GABA

receptor α

in perfused brain tissue was detected with immunohistochemistry. Results The body weight was significantly lower

the susceptibility to convulsion and the excitability of pyramidal neurons in hippocampal CA1 were higher

and the expression level of hippocampal GABA

receptor α

in brain tissue was lower in 3-h isolation group than in normal group. However

no significant difference was found in the above parameters between 15-min isolation group and normal group. Conclusion Stress due to 3-h mother and infant isolation may produce adverse effects on the growth and development

and susceptibility to epilepsy of infantile rats. Increased excitability of pyramidal neurons in hippocampal CA1 and decreased expression of hippocampal GABA

receptor α

in brain tissue may be one of the potential mechanisms.

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Related Institution

Experimental Center of Basic Medicine, College of Basic Medical Sciences， Army Medical University （Third Military Medical University）

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