ERK/NF-κB signal pathway regulates bilirubin-induced apoptosis of hippocampal neurons in vitro

王少雯; 华子瑜

您当前的位置：

首页 >

文章列表页 >

ERK/NF-κB signal pathway regulates bilirubin-induced apoptosis of hippocampal neurons in vitro

更新时间：2026-01-09

- ERK/NF-κB signal pathway regulates bilirubin-induced apoptosis of hippocampal neurons in vitro
- Journal of Army Medical University Vol. 32, Issue 16, Pages: 1754-1757(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.81;R329.26;R329.28
- Published：2010
- 稿件说明：
移动端阅览
王少雯,华子瑜.细胞外信号调节蛋白激酶/核转录因子-κB调控胆红素诱导的海马神经细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(16):1754-1757.

　Wang Shaowen,Hua Ziyu.ERK/NF-κB signal pathway regulates bilirubin-induced apoptosis of hippocampal neurons in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(16):1754-1757.
王少雯,华子瑜.细胞外信号调节蛋白激酶/核转录因子-κB调控胆红素诱导的海马神经细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(16):1754-1757. DOI：

　Wang Shaowen,Hua Ziyu.ERK/NF-κB signal pathway regulates bilirubin-induced apoptosis of hippocampal neurons in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(16):1754-1757. DOI：

摘要

目的探讨细胞外信号调节蛋白激酶（extracellular signalregulated protein kinase，ERK）1/2和核转录因子-κB（NF-κB）在胆红素诱导的海马神经细胞凋亡过程中的作用。方法将体外培养的海马神经细胞分为处理组（胆红素处理神经元）、抑制组（胆红素+ERK1/2抑制剂PD98059）以及对照组（DMSO）。采用改良噻唑蓝比色法（MTT法）和Hoechst33342DNA染色法，观察各组细胞存活率及形态学特征；免疫细胞化学检测NF-κB蛋白的表达。结果处理组细胞存活率（85.418±1.406）%明显低于对照组（99.990±2.782）%（P0.01），而高于抑制组(63.951±1.148）%（P0.01）；对照组和抑制组NF-κB蛋白光密度值、阳性细胞率[分别为（0.157±0.037）、（0.986±0.795）%和（0.249±0.059）、（2.622±1.552）%]均明显低于处理组[分别为（0.306±0.072）、（6.882±2.626）%，P0.01]。结论胆红素可激活原代培养的海马神经细胞ERK/NF-κB信号转导通路；抑制ERK1/2通路可抑制NF-κB活性而加剧胆红素的神经细胞毒性。

Abstract

Objective To investigate the role of extracellular signal - regulated protein kinase (ERK)1/2 and nuclear transcription factors (NF - κ B) in the process of bilirubin - induced apoptosis of hippocampal neurons. Methods Hippocampal neurons cultured

in vitro

were divided into treated group (bilirubin)

inhibited group (PD98059 and then bilirubin) and control group (DMSO). Neuronal viability and morphology were tested by modified MTT assay and fluorescent staining with Hoechst33342. The expression of NF

- κ B protein in hippocampal neurons were detected by immunocytochemistry. Results Neuronal viability in treated group [(85.418 ± 1.406)%

]

was significantly decreased compared to that of control group [(99.990 ± 2 . 782)%

0 . 01

]

but higher than that in inhibited group [(63.951 ± 1.148)%

0 . 01

]

. Moreover

the light density value and positive cell rate of NF - κ B protein in control group were (0.157 ± 0.037) and (0.986 ± 0 . 795)%

respectively

whereas those in inhibited group were (0.249 ± 0.059) and (2.622 ± 1.552)%

both of which were lower than those of treated group [(0.306 ± 0.072) and (6.882 ± 2.626)%

]

respectively (

0 . 01). Conclusion ERK/NF - κ B protein signal pathway of hippocampal neuron

in vitro

is activated by bilirubin

and inhibition of ERK/NF - κ B pathway deteriorates neuron apoptosis induced by bilirubin.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Defective hippocampal astrocyte and neuronal primary cilia function in offspring brain of pregnant rats with advanced age might be associated with promoting apoptosis through LKB1/AMPK pathway

Dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia

Effect of propranolol on retention of fear memory and morphology of neurons in hippocampal CA3 area of rats with conditioned fear

Phosphorylized ERK1/2 is upregulated in diabetic rats after global cerebral-ischemia reperfusion injury

Protective effects of hypoxia preconditioning on rat brain against hypobaric hypoxia

Related Author

韩子瑶

罗涵予

桂健雄

袁萍

蒋莉

邹哲华

陶陶

徐坚

Related Institution

Department of Neurology Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Immunology, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Laboratory Animal Science， College of Basic Medical Sciences, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Pharmaceutical Analysis, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

⁰