Expression of CD82/KAI1 in glioma cell lines and its effect on cell migration

陈亮; 罗文萍; 钟东; 王亮; 唐兆华; 杨刚; 唐文渊

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Expression of CD82/KAI1 in glioma cell lines and its effect on cell migration

更新时间：2026-01-09

- Expression of CD82/KAI1 in glioma cell lines and its effect on cell migration
- Journal of Army Medical University Vol. 32, Issue 13, Pages: 1438-1441(2010)
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  CLC： R730.264;R394.2;R73-37
- Published：2010
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陈亮,罗文萍,钟东,王亮,唐兆华,杨刚,唐文渊.CD82/KAI1在神经胶质瘤细胞株中的表达及对细胞迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1438-1441.

　Chen liang,Luo Wenping,Zhong Dong,Wang Liang,Tang Zhaohua,Yang Gang,Tang Wenyuan.Expression of CD82/KAI1 in glioma cell lines and its effect on cell migration[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1438-1441.
陈亮,罗文萍,钟东,王亮,唐兆华,杨刚,唐文渊.CD82/KAI1在神经胶质瘤细胞株中的表达及对细胞迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1438-1441. DOI：

　Chen liang,Luo Wenping,Zhong Dong,Wang Liang,Tang Zhaohua,Yang Gang,Tang Wenyuan.Expression of CD82/KAI1 in glioma cell lines and its effect on cell migration[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1438-1441. DOI：

摘要

目的研究CD82/KAI1在胶质瘤CHG-5及U251细胞株的表达及意义。方法 RT-PCR、免疫细胞化学、免疫荧光检测CHG-5及U251细胞中CD82/KAI1的表达；分别设立1∶100、1∶200 CD82/KAI1抗体干预组和空白对照组，通过划痕实验对CD82/KAI1对细胞迁移能力的影响进行分析。结果 ①U251细胞中CD82/KAI1的mRNA表达高于CHG-5细胞(P0.01)；U251细胞中CD82/KAI1的蛋白水平表达高于CHG-5细胞(P＜0.01)。②CD82/KAI1抗体干预组迁移细胞数量均显著高于对照组(P0.05)，且迁移细胞数量随抗体浓度升高而增加(P0.05)。结论 CD82/KAI1在恶性胶质瘤细胞株中表达，且在U251细胞中表达较CHG-5细胞高。CD82/KAI1可抑制胶质瘤U251细胞的迁移。

Abstract

Objective To study the CD82/KAI1 expression in glioma cell lines

CHG-5 and U251. Methods CD82/KAI1 expression in CHG-5 and U251 was detected by RT-PCR

immunocytochemistry

and immunofluorescence

respectively. CD82/KAI1 antibodies

diluted at 1∶100 and 1∶200

served as an intervention group and a blank control group

respectively. Effect of CD82/KAI1 expression on cell migration was analyzed by scratch test. Results The expression level of CD82/KAI1 mRNA and protein was higher in U251 than in CHG-5 (P0.01). The number of migrated cells was greater in CD82/KAI1 antibody intervention group than in blank control group (P0.05)

and increased with the increasing antibody concentration. Conclusion CD82/KAI1 is expressed in glioma cell lines

with its expression level higher in U251 than in CHG-5. CD82/KAI1can suppress the migration of glioma U251 cells.

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