Triptolide induces apoptosis of Hut102 cells through p38MAPK pathway

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Triptolide induces apoptosis of Hut102 cells through p38MAPK pathway

更新时间：2026-01-09

- Triptolide induces apoptosis of Hut102 cells through p38MAPK pathway
- Journal of Army Medical University Vol. 32, Issue 19, Pages: 2109-2111(2010)
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  CLC： R285.5;R730.23;R733.1
- Published：2010
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谢会霞,郝进.雷公藤内酯醇通过p38MAPK信号通路诱导Hut102细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(19):2109-2111.

　Xie Huixia,Hao Jin.Triptolide induces apoptosis of Hut102 cells through p38MAPK pathway[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(19):2109-2111.
谢会霞,郝进.雷公藤内酯醇通过p38MAPK信号通路诱导Hut102细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(19):2109-2111. DOI：

　Xie Huixia,Hao Jin.Triptolide induces apoptosis of Hut102 cells through p38MAPK pathway[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(19):2109-2111. DOI：

摘要

目的　探讨雷公藤内酯醇（triptolide

TP）诱导皮肤T细胞淋巴瘤蕈样肉芽肿细胞株Hut102的凋亡及其机制。　方法　体外培养的Hut102细胞与12.5、25、50、100 nmol/L雷公藤内酯醇共同孵育，分别在24、48、72 h用MTT法检测Hut102细胞的增殖抑制率，Annexin V-FITC/PI 双染流式细胞术分析细胞凋亡，Western blot检测Hut102细胞中磷酸化p38丝裂原活化蛋白激酶(p-p38)、caspase-3蛋白的表达水平。　结果　不同浓度雷公藤内酯醇（12.5、25、50、100 nmol/L）作用Hut102细胞24 h后的增殖抑制率分别为8.67%、30.02%、52.54%、59.62%。Annexin V-FITC/PI 双染流式细胞术检测经25、50、100 nmol/L雷公藤内酯醇处理24 h后Hut102细胞凋亡率分别为9.19%、21.49%、26.34%，当50 nmol/L雷公藤内酯醇组预先用p38MAPK特异性抑制剂SB203580干预后，Hut102细胞凋亡率下降为14.35%。不同浓度雷公藤内酯醇（50、100 nmol/L）处理Hut102细胞后p38、caspase-3蛋白的表达被激活，同样50 nmol/L雷公藤内酯醇组也预先用p38MAPK特异性抑制剂SB203580干预，结果p-p38的表达降低，caspase-3激活被抑制。　结论　雷公藤内酯醇可以抑制Hut102细胞的增殖，其作用机制可能是通过激活p38MAPK信号通路使部分Hut102细胞产生凋亡。

Abstract

Objective To study the effect of triptolide on apoptosis of human cutaneous T-cell lymphoma Hut102 cells and its mechanism. Methods Cutaneous T-cell lymphoma cell line Hut102 was co-cultured with triptolide solution at the terminal concentration of 12.5

25

50 and 100 nmol/L

respectively

in 96 well plates．At 24，48，and 72 h after co-culture，inhibitory effect of triptolide on Hut102 cells was detected by MTT assay

apoptosis rate of Hut102 cells was assessed by Annexin V-FITC/PI

and expression of phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK ) and caspase-3 was determined by Western blot analysis. Results Triptolide inhibited the proliferation of Hut102 cells in a concentration-and time-dependent manner. Annexin V-FITC/PI showed that the apoptotic rate of Hut102 cells was 9.19%，21.49% and 26.34%

respectively

in 24 h after they were treated with triptolide at the concentration of 25

50 and 100 nmol/L

respectively．However，the apoptosis rate of Hut102 cells was decreased by 14.35% after they were interfered with the p38MAPK inhibitor (SB203580). The expression of p-p38MAPK and caspase-3 was activated in Hut102 cells by different concentrations of triptolide (50 and 100 nmol/L). However

the expression of phosphorylated p38MAPK was decreased and the activity of caspase-3 was inhibited by triptolide at the concentration of 50 nmol/L interfered with SB203580. Conclusion Triptolide can inhibit the proliferation of Hut102 cells by activating the p38MAPK signal pathway

thus inducing apoptosis of certain Hut102 cells．

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