Relationship of NF-κB expression with vascular proliferation in basilar artery of subarachnoid hemorrhage rabbits

张建忠; 周政; 刘俊; 杨辉; 黄巍; 张旋; 冯宝海

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Relationship of NF-κB expression with vascular proliferation in basilar artery of subarachnoid hemorrhage rabbits

更新时间：2026-01-09

- Relationship of NF-κB expression with vascular proliferation in basilar artery of subarachnoid hemorrhage rabbits
- Journal of Army Medical University Vol. 32, Issue 18, Pages: 1976-1980(2010)
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  CLC： R743.35;R322.121;R329.25
- Published：2010
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张建忠,周政,刘俊,杨辉,黄巍,张旋,冯宝海.NF-κB在兔蛛网膜下腔出血模型基底动脉中的表达及其与血管平滑肌增殖的关系[J].陆军军医大学学报(原第三军医大学学报),2010,32(18):1976-1980.

　Zhang Jianzhong,Zhou Zheng,Liu Jun,Yang Hui,Huang Wei,Zhang Xuan,Feng Baohai.Relationship of NF-κB expression with vascular proliferation in basilar artery of subarachnoid hemorrhage rabbits[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(18):1976-1980.
张建忠,周政,刘俊,杨辉,黄巍,张旋,冯宝海.NF-κB在兔蛛网膜下腔出血模型基底动脉中的表达及其与血管平滑肌增殖的关系[J].陆军军医大学学报(原第三军医大学学报),2010,32(18):1976-1980. DOI：

　Zhang Jianzhong,Zhou Zheng,Liu Jun,Yang Hui,Huang Wei,Zhang Xuan,Feng Baohai.Relationship of NF-κB expression with vascular proliferation in basilar artery of subarachnoid hemorrhage rabbits[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(18):1976-1980. DOI：

摘要

目的观察兔蛛网膜下腔出血(subarachnoid hemorrhage，SAH)模型基底动脉中核转录因子（nuclear factor-κB，NF-κB)和增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）的时间动态表达情况，并分析NF-κB与SAH诱导的基底动脉壁增殖的关系。方法 40只雄性新西兰大白兔完全随机分为假手术组和SAH组，每组20只，每组各分为术后1、4、7、14 d亚组（n=5）。SAH组采用枕大池二次注血法建立脑血管痉挛动物模型，假手术组注入等量生理盐水，各组于二次注血术后第1、4、7、14天观察基底动脉组织病理学改变，并应用免疫组化、Western blot法分析各组基底动脉中NF-κB、PCNA 阳性细胞比值和蛋白水平表达的差异。结果与假手术组相比，SAH组基底动脉血管壁平滑肌随观察时间逐渐增厚，管腔狭窄，在第7天达高峰(P0.05)，在第14天时恢复至正常水平；NF-κB和PCNA蛋白表达随时间逐渐增强，在第7天达高峰(P0.05)，在第14天时表达下降。NF-κB的表达与基底动脉平滑肌厚度变化及PCNA表达变化呈显著正相关(r=0.80、r=0.75， P0.05）。结论 NF-κB的激活可能参与了SAH后脑血管平滑肌细胞增殖，NF-κB可能通过转录多种促血管增殖因子参与SAH诱导的血管壁增殖过程。

Abstract

Objective To investigate the dynamic expressions of nuclear transcription factor nuclear factor-κB (NF-κB) and the dynamic profile of proliferating cell nuclear antigen (PCNA) in the basilar artery of rabbit model of subarachnoid hemorrhage (SAH)

and explore the relationship of NF-κB and basilar artery wall proliferation. Methods Forty New Zealand male rabbits were randomly divided into Sham group and 4 SAH groups according to different time points (n=5). Animal models of cerebral vasospasm was established by double injections of fresh autologous arterial blood into the cisterna magna. The animals of sham group received an injection of normal saline as same volume. In 1

7 and 14 d after second injection

the rabbits were killed and the basilar artery was taken out. Expressions of NF-κB and PCNA of basilar artery were determined by immunohistochemical staining and Western blot analysis. Morphological changes of rabbit basilar artery were observed after HE staining. Results Compared with Sham group

the smooth muscle layer of basilar artery wall in SAH group were gradually thickening

and the lumen became stenosed during the observation period

and these phenomena peaked on the 7th day (P0.05) and restored to normal conditions on the 14th day. The protein expression of NF-κB of basilar artery was markedly higher in SAH rabbits with time elapse

and reached the peak on 7th day (P0.05)

however

it was decreased significantly on 14th day. Interestingly

the expression of PCNA in the basilar artery was in coincidence with that of NFκB in SAH rabbits. Moreover

the expression of NF-κB protein was positively correlated with the thickening of smooth muscle wall and the expression of PCNA protein (r=0.80

r=0.75

P0.01). Conclusion Activation of NF-κB may be involved in the proliferation of cerebral vascular smooth muscle cells after SAH. NF-κB might take part in vascular wall thickening after SAH via transcripting multiple vascular proliferation factors.

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