Effect of adenovirus-mediated NgR specific RNA interference on neuroethology of rats with experimental autoimmune encephalomyelitis

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Effect of adenovirus-mediated NgR specific RNA interference on neuroethology of rats with experimental autoimmune encephalomyelitis

更新时间：2026-01-09

- Effect of adenovirus-mediated NgR specific RNA interference on neuroethology of rats with experimental autoimmune encephalomyelitis
- Journal of Army Medical University Vol. 32, Issue 21, Pages: 2318-2321(2010)
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  CLC： R392.3;R741.05;R744.3
- Published：2010
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张艳,秦新月,王敬,张沁丽.腺病毒介导的NgR特异性RNA干扰对实验性自身免疫性脑脊髓炎大鼠神经行为学的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(21):2318-2321.

　Zhang Yan,Qin Xinyue,Wang Jing,Zhang Qinli.Effect of adenovirus-mediated NgR specific RNA interference on neuroethology of rats with experimental autoimmune encephalomyelitis[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(21):2318-2321.
张艳,秦新月,王敬,张沁丽.腺病毒介导的NgR特异性RNA干扰对实验性自身免疫性脑脊髓炎大鼠神经行为学的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(21):2318-2321. DOI：

　Zhang Yan,Qin Xinyue,Wang Jing,Zhang Qinli.Effect of adenovirus-mediated NgR specific RNA interference on neuroethology of rats with experimental autoimmune encephalomyelitis[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(21):2318-2321. DOI：

摘要

目的观察NgR特异性RNA干扰的重组腺病毒(Ad-shRNA-NgR)转染实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis

EAE）大鼠脑组织后，EAE大鼠脑内NgR表达及神经行为学的情况。方法 Wistar大鼠108只，采用随机数字表法分为正常对照组(n=27)、空白对照组(n=27)、阴性对照组(n=27)、特异性RNAi组(n=27)。造模后第7天，通过侧脑室对阴性对照组及特异性RNAi组分别注射Ad-shRNA-HK、Ad-shRNA-NgR，空白对照组以等量的PBS代替。注射后第7、14天，RT-PCR、免疫荧光染色观察脑组织中NgR的表达并行Koh神经功能评分。结果特异性RNAi组大鼠脑组织中NgR mRNA的表达及NgR免疫荧光染色的IOD值：注射后第7天，较空白及阴性对照组均明显下降（P0.01）；注射后第14天，较该组注射后第7天时增高（P0.05），但仍低于空白及阴性对照组（P0.05）。特异性RNAi组EAE的发病潜伏期延长（P0.05），Koh神经功能评分降低（P0.05），体质量无明显减轻。结论腺病毒介导的NgR特异性RNAi可明显下调EAE大鼠脑组织中NgR的表达，改善EAE大鼠的神经行为。

Abstract

Objective To observe the expression of NgR in the brain and the neuroethology of rats with experimental autoimmune encephalomyelitis(EAE) after Ad-shRNA-NgR was transfected into the lateral cerebral ventricle of rats. Methods One hundred and eight Wistar rats were randomly divided into normal control group

blank control group

negative control group

and specific RNAi group

27 rats in each group. Rats in blank control group

negative control group

and specific RNAi group were injected with PBS

Ad-shRNA-HK and Ad-shRNA-NgR

respectively

in 7 d after EAE model establishment. Expression of NgR in brain tissue of rats was detected by RT-PCR with immunofluorescence staining after 7 and 14 d

respectively. Neurologic function was scored according to the Koh scoring system. Results The expression of NgR mRNA and the IOD of NgR in specific RNAi group was significantly lower than the blank or negative groups (P0.01) at the 7th day

and was higher in 14 d than in 7 d after injection of different substances (P0.05) and was still lower than in blank or negative group (P0.05) . The latent period of EAE was longer and the neurologic score was lower in specific RNAi group than in blank or negative group（P0.05）. No significant change was observed in body weight of rats. Conclusion Adenovirus-mediated NgR specific RNAi can down-regulate the expressing of NgR and ameliorate the neural behaviors of rats with EAE.

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