p38MAPK signal pathway modulates proliferation, apoptosis and cell cycle of rat hepatic stellate cells stimulated by acetaldehyde

叶平; 杨波; 唐文; 卓强; 郑人源; 蒋明德

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p38MAPK signal pathway modulates proliferation, apoptosis and cell cycle of rat hepatic stellate cells stimulated by acetaldehyde

更新时间：2026-01-09

- p38MAPK signal pathway modulates proliferation, apoptosis and cell cycle of rat hepatic stellate cells stimulated by acetaldehyde
- Journal of Army Medical University Vol. 32, Issue 20, Pages: 2197-2200(2010)
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  CLC： R322.47;R329.28;R363.13
- Published：2010
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叶平,杨波,唐文,卓强,郑人源,蒋明德.p38MAPK信号通路对乙醛刺激的大鼠肝星状细胞增殖、凋亡及细胞周期的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(20):2197-2200.

　Ye Ping,Yang Bo,Tang Wen,Zhuo Qiang,Zheng Renyuan,Jiang Mingde.p38MAPK signal pathway modulates proliferation, apoptosis and cell cycle of rat hepatic stellate cells stimulated by acetaldehyde[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(20):2197-2200.
叶平,杨波,唐文,卓强,郑人源,蒋明德.p38MAPK信号通路对乙醛刺激的大鼠肝星状细胞增殖、凋亡及细胞周期的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(20):2197-2200. DOI：

　Ye Ping,Yang Bo,Tang Wen,Zhuo Qiang,Zheng Renyuan,Jiang Mingde.p38MAPK signal pathway modulates proliferation, apoptosis and cell cycle of rat hepatic stellate cells stimulated by acetaldehyde[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(20):2197-2200. DOI：

摘要

目的观察用特异性p38丝裂原活化蛋白激酶抑制剂SB203580阻断p38MAPK信号通路对乙醛刺激的大鼠肝星状细胞（hepatic stellate cell，HSC）增殖、凋亡及细胞周期的影响。方法体外培养大鼠HSC株，用SB203580（浓度为5、10、20 μmol /L）对乙醛（浓度200 μmol /L）刺激的大鼠HSC进行干预，分别以酶标仪及流式细胞仪检测HSC增殖、凋亡及周期的变化。结果不同浓度SB203580对HSC增殖有抑制作用，增殖抑制率分别为21.6%、27.0%、31.5%，与乙醛对照组有显著差异(P0.05)，且呈剂量效应关系；对HSC凋亡有促进作用，凋亡率分别为（13.7±0.3）%、(14.9±0.2)%、(16.1±0.2)%，凋亡率逐渐增加，与乙醛对照组有显著差异(P0.05)，且呈剂量效应关系；可以明显阻滞乙醛刺激的HSC由G

期进入S期，G

期细胞比例逐渐增高[(29.1±1.9)%、(35.5±3.4)%、(46.0±2.6)%

]

，S期细胞比例逐渐降低[（53.4±2.6）%、（48.4±3.6）%、（43.8±1.1）%

]

，与乙醛对照组有显著差异(P0.05)，呈剂量效应关系。结论 SB203580特异性阻断p38MAPK信号通路后，乙醛刺激的HSC增殖受到抑制，凋亡得到促进，细胞周期由G

期进入S期受到阻滞。

Abstract

Objective To investigate the effects of blocking p38MAPK by its specific blocker

SB203580

on the proliferation

apoptosis and cell cycle of rat hepatic stellate cells (HSCs). Methods Rat HSCs stimulated by acetaldehyde (200 μmol/L) were incubated with SB203580 at concentrations of 5

10 and 20 μmol/L respectively. Cellular proliferation inhibition rate (CPIR) were ana

lyzed by MTT assay

and the changes of apoptotic rate and cell cycle were analyzed by flow cytometry. Results Compared with acetaldehyde control group

SB203580 at different concentrations inhibited the proliferation of HSC significantly and the CPIR was 21.6%

27.0% and 31.5% respectively (P0.05). SB203580 also promoted the apoptosis of HSC significantly with the apoptotic rate of (13.7±0.3)%

(14.9±0.2)%

and (16.1±0.2)% respectively (P0.05). And the cell percentage of G

was significantly increased by (29.1±1.9)%

(35.5±3.4)% and (46.0±2.6)% respectively (P0.05)

while

cell percentage of S was significantly decreased by (53.4±2.6)%

(48.4±3.6)% and (43.8±1.1)%(P0.05). Conclusion After p38MAPK is blocked by SB203580 specifically in HSC stimulated by acetaldehyde

cell proliferation is inhibited but apoptosis promoted

and the cell cycle from G

to S is arrested.

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