ITGB4BP downregulates activity of Wnt/β-catenin signaling pathway in HEK293 cells

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ITGB4BP downregulates activity of Wnt/β-catenin signaling pathway in HEK293 cells

更新时间：2026-01-09

- ITGB4BP downregulates activity of Wnt/β-catenin signaling pathway in HEK293 cells
- Journal of Army Medical University Vol. 32, Issue 18, Pages: 1945-1948(2010)
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  CLC： R394-33;R341;R394.3
- Published：2010
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赖庆梧,彭旭,谭江琳,张小容,胡小红,张毅,罗高兴,吴军.ITGB4BP对Wnt/β-catenin信号通路活性的下调作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(18):1945-1948.

　Lai Qingwu,Peng Xu,Tan Jianglin,Zhang Xiaorong,Hu Xiaohong,Zhang Yi,Luo Gaoxing,Wu Jun.ITGB4BP downregulates activity of Wnt/β-catenin signaling pathway in HEK293 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(18):1945-1948.
赖庆梧,彭旭,谭江琳,张小容,胡小红,张毅,罗高兴,吴军.ITGB4BP对Wnt/β-catenin信号通路活性的下调作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(18):1945-1948. DOI：

　Lai Qingwu,Peng Xu,Tan Jianglin,Zhang Xiaorong,Hu Xiaohong,Zhang Yi,Luo Gaoxing,Wu Jun.ITGB4BP downregulates activity of Wnt/β-catenin signaling pathway in HEK293 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(18):1945-1948. DOI：

摘要

目的探讨β4整合素结合蛋白ITGB4BP 对Wnt/β-catenin信号通路的影响。方法将对照组空载体pCMV-3B质粒和实验组pCMV-ITGB4BP质粒分别转染至HEK293细胞中，用Western blot和激光共聚焦技术检测细胞中β-catenin含量的变化，用含TCF/LEF启动子的荧光素酶TOPFlash质粒和内参pRL-TK质粒共转染对照组和实验组的HEK293细胞，用荧光素酶活性分析法检测ITGB4BP转染后HEK293细胞TCF/LEF活性的变化。设不转染质粒组为正常组，对照组和实验组均分别设未加LiCl组和加LiCl组。结果 Western blot检测结果：与正常组或对照组比较，实验组细胞β-catenin表达显著减少（P0.05）。加LiCl之后，β-catenin的表达显著增强（P0.05），与未加有LiCl的正常组或对照组比较，加LiCl的实验组细胞内β-catenin仍有较高的表达，但差异无显著性。激光共聚焦技术观察：实验组β-catenin（绿色荧光）表达较对照组降低。加LiCl之后，β-catenin（绿色荧光）表达增强，与未加LiCl的对照组相比，加LiCl的实验组仍有较强的荧光强度。荧光素酶活性分析法检测：实验组的荧光素酶活性较对照组显著降低（P0.05）。LiCl作用之后，Wnt/β-catenin信号通路活性明显增强（P0.05），与未加LiCl的对照组相比，加LiCl的实验组仍有较强的荧光素酶活性（P0.05）。结论 ITGB4BP可以下调Wnt/β-catenin信号通路的活性。

Abstract

Objective To investigate the effects of integrin beta 4 binding protein (ITGB4BP) on the Wnt/β-catenin signaling pathway. Methods Human embryonic kidney cell line HEK293 was transfected with blank vector plasmid pCMV-3B as the control group and plasmid pCMV-ITGB4BP as the experiment group

respectively. β-catenin expression was checked by Western blot analysis and laser confocal microscopy. After the 2 groups were cotransfected with plasmid TOPFlash containing promoter TCF/LEF and plasmid pRL-TK as the internal control

the TCF/LEF activity was analyzed by luciferase activity assay. The group transfected with no other plasmid was set as the normal group. Both the control group and the experiment group were treated with LiCl (40 mmol/L) or not in 32 h after transfection. Results Western blotting showed that

as compared with the normal group and the control group

β-catenin level was decreased significantly in the experiment group (P0.05)

but the level was increased significantly after treatment with LiCl (P0.05). As compared with the normal group and the control group without treated with LiCl

β-catenin level was still highly expressed in the experiment group treated with LiCl

but not increased significantly. Laser confocal microscopy showed that the expression of β-catenin (flagged with green fluorescence) was decreased compared with the control group. After treatment with LiCl

the expression of β-catenin was increased. As compared with the control group without treated with LiCl

the fluorescence intensity of β-catenin was strengthened in the experiment group treated with LiCl. Luciferase activity assay showed that

as compared with the control group

the TCF/LEF activity was reduced significantly (P0.05) in the experiment group. After treatment with LiCl

the activity of Wnt/β-catenin signaling pathway was strengthened significantly (P0.05). As compared with the control group without treated with LiCl

the activity of luciferase was strengthened in the experiment group treated with LiCl (P0.05). Conclusion ITGB4BP can downregulate the activity of Wnt/β-catenin signaling pathway.

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