TLR4 and MD2 antisense genes suppress activation of alveolar typeⅡepithelial cells induced by lipopolysaccharide

李永旺; 麻莉; 张德明; 钱桂生

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TLR4 and MD2 antisense genes suppress activation of alveolar typeⅡepithelial cells induced by lipopolysaccharide

更新时间：2026-01-09

- TLR4 and MD2 antisense genes suppress activation of alveolar typeⅡepithelial cells induced by lipopolysaccharide
- Journal of Army Medical University Vol. 32, Issue 12, Pages: 1271-1274(2010)
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  CLC： R322.35;R392-33;R392.2
- Published：2010
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李永旺,麻莉,张德明,钱桂生.TLR4及MD2反义基因对内毒素诱导的肺泡Ⅱ型细胞活化的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1271-1274.

　Li Yongwang,Ma Li,Zhang Deming,Qian Guisheng.TLR4 and MD2 antisense genes suppress activation of alveolar typeⅡepithelial cells induced by lipopolysaccharide[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1271-1274.
李永旺,麻莉,张德明,钱桂生.TLR4及MD2反义基因对内毒素诱导的肺泡Ⅱ型细胞活化的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1271-1274. DOI：

　Li Yongwang,Ma Li,Zhang Deming,Qian Guisheng.TLR4 and MD2 antisense genes suppress activation of alveolar typeⅡepithelial cells induced by lipopolysaccharide[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1271-1274. DOI：

摘要

目的观察Toll样受体4(Toll-like receptor 4

TLR4)及myeloid differentiation protein 2（MD2）反义基因对内毒素（lipopolysaccharide，LPS）诱导的肺泡Ⅱ型细胞NF-κΒ活化的影响。方法培养的肺泡Ⅱ型细胞分为：正常细胞（对照）组、LPS组、LPS+转染空载体组、LPS+转染TLR4反义基因组、LPS+转染MD2反义基因组、LPS+转染TLR4-MD2反义基因组（n=8）。Northern blot检测转染细胞的TLR4 及MD2 mRNA表达，Western blot检测转染细胞TLR4及MD2蛋白表达，电泳迁移率改变法检测 NF-κΒ的活性，ELISA测定细胞培养上清中TNF-α和IL-6含量。结果与对照组比较，LPS刺激后的细胞TLR4及MD2 mRNA和蛋白表达、NF-κΒ活性、TNF-α和IL-6的生成均显著增加（P0.01）；而转染反义基因组细胞与其他LPS刺激组比较，TLR4 及MD2 mRNA和蛋白的表达、NF-κΒ活性及TNF-α和IL-6的含量均明显降低（P＜0.01）。结论 TLR4及MD2反义基因能有效抑制LPS诱导的肺泡Ⅱ型细胞的活化。

Abstract

Objective To observe effects of the antisense genes of Toll-like receptor 4 (TLR4 ) and myeloid differentiation protein-2 (MD2) on the activation of NF-κΒ in alveolar typeⅡepithelial cells (ATⅡcells) induced by lipopolysaccharide (LPS). Methods Cultured ATⅡcells were randomized to normal cells (control) group

LPS group

LPS+empty vector group

LPS+TLR4 antisense gene group

LPS+MD2 antisense gene group

LPS+TLR4-MD2 antisense gene group. The expressions of TLR4 and MD2 mRNA and protein in ATⅡcells were detected by Northern blotting and Western blotting respectively. The activity of NF-κΒ in ATⅡcells was measured with electrophoretic mobility shift assay (EMSA). The TNF-α and IL-6 concentrations in the supernatant of cultured ATⅡcells were tested with ELISA． Results Compared with control groups

the expressions of TLR4 and MD2 mRNA and protein

the NF-κΒ activity

the levels of TNF-α and IL-6 were increased remarkably in LPS group and LPS+empty vector group (P0.01). After stimulating with the antisense genes

the expressions of TLR4 and MD2 mRNA and protein

the NF-κΒ activity

the levels of TNF-α and IL-6 were decreased remarkably than in LPS and LPS+empty vector group (P0.01). Conclusion The antisense gene of TLR4 and MD2 can inhibit activation of ATⅡ cells induced by LPS.

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