Silencing of osteopontin suppresses invasion and apoptosis of human gastric cancer MKN28 cells

王霆; 郭传勇

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Silencing of osteopontin suppresses invasion and apoptosis of human gastric cancer MKN28 cells

更新时间：2026-01-09

- Silencing of osteopontin suppresses invasion and apoptosis of human gastric cancer MKN28 cells
- Journal of Army Medical University Vol. 32, Issue 14, Pages: 1524-1528(2010)
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  CLC： R73-362;R730.23;R735.2
- Published：2010
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王霆,郭传勇.OPN基因沉默抑制MKN28细胞生长、侵袭及凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(14):1524-1528.

　Wang Tin,Guo Chuanyong.Silencing of osteopontin suppresses invasion and apoptosis of human gastric cancer MKN28 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(14):1524-1528.
王霆,郭传勇.OPN基因沉默抑制MKN28细胞生长、侵袭及凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(14):1524-1528. DOI：

　Wang Tin,Guo Chuanyong.Silencing of osteopontin suppresses invasion and apoptosis of human gastric cancer MKN28 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(14):1524-1528. DOI：

摘要

目的检测骨桥蛋白（osteopontin，OPN）对人胃癌细胞MKN28生长、侵袭及凋亡的影响。方法利用脂质体转染的方法，进行胃癌转移相关基因骨桥蛋白（OPN）的siRNA，并通过荧光定量PCR以及免疫荧光的方法，进行siRNA效果鉴定。根据siRNA的抑制效果，将细胞分为正常对照组、非特异干扰组、特异干扰24 h组、特异干扰36 h组和特异干扰48 h组，比较对照组与不同siRNA作用时间组的细胞增殖、细胞凋亡、迁移黏附能力的改变。结果 siRNA 作用48 h组，OPN的表达最低；相应地，其细胞活力降低，增殖抑制率达30.20%，迁移黏附能力降低，基质膜上的细胞数目明显减少[对照组与OPN干扰组比较：（42.0±9.4） vs （21.8±6.9），P0.01]，细胞对基底膜基质和纤维连接蛋白的黏附百分比明显降低[对照组与OPN干扰组比较：基底膜基质，（41.5±8.4）% vs （20.5±4.5）%；纤维连接蛋白，（25.3±4.5）% vs （14.6±2.5）%]，凋亡增加。结论 OPN基因沉默能有效抑制人胃癌细胞MKN28的生长、侵袭，并促进凋亡的发生。

Abstract

Objective To detect the effect of osteopontin (OPN) siRNA on the growth

invasion and apoptosis of human gastric cancer cell line MKN28. Methods The eukaryotic expression vector of siRNA specific for OPN was constructed by liposome transfection

and the silencing effect was identified by fluorescence quantitative PCR and immunofluorescence staining. According to the inhibitory effect of the siRNA vectors

the experiment was divided into control group

non-specific OPN siRNA group

24-hour specific OPN siRNA group

36-hour specific OPN siRNA group

and 48-hour specific OPN siRNA group. The cell proliferation

apoptosis

migration and adhesion was finally detected among control group and OPN siRNA groups at different time points. Results For 48-hour specific OPN siRNA group

the expression of OPN in MKN28 cells was the lowest. Accordingly

the cell viability was decreased and the cell proliferation inhibitory rate was reduced to 30.20%; the ability of migration and adhesion was decreased

the number of cells attached on stromal cell membrane was much lesser (21.8±6.9 vs 42.0±9.4 in control group)

and the ratio of cell adhesion on basement membrane matrix and fibronectin was lower [basement membrane matrix: (41.5±8.4)% vs (20.5±4.5)%; fibronectin: (25.3±4.5)% vs (14.6±2.5)% in control group]

as well as the cell apoptotic rate was increased compared with the control group. Conclusion OPN gene silencing can inhibit the growth and invasion

as well as accelerate apoptosis in human gastric cancer MKN28 cells.

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