Effect of Notch-1 on expression of NGF in olfactory ensheathing cells of rats

卓飞; 孙善全; 余维华; 刘辉; 邱国平; 杨美

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Effect of Notch-1 on expression of NGF in olfactory ensheathing cells of rats

更新时间：2026-01-09

- Effect of Notch-1 on expression of NGF in olfactory ensheathing cells of rats
- Journal of Army Medical University Vol. 32, Issue 21, Pages: 2290-2293(2010)
- 作者机构：
- 作者简介：
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- DOI：
  CLC： R741.05;R322.81;R329.25;R394.2
- Published：2010
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卓飞,孙善全,余维华,刘辉,邱国平,杨美.Notch-1对大鼠嗅球成鞘细胞神经生长因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(21):2290-2293.

　Zhuo Fei,Sun Shanquan,Yu Weihua,Liu Hui,Qiu Guoping,Yang Mei.Effect of Notch-1 on expression of NGF in olfactory ensheathing cells of rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(21):2290-2293.
卓飞,孙善全,余维华,刘辉,邱国平,杨美.Notch-1对大鼠嗅球成鞘细胞神经生长因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(21):2290-2293. DOI：

　Zhuo Fei,Sun Shanquan,Yu Weihua,Liu Hui,Qiu Guoping,Yang Mei.Effect of Notch-1 on expression of NGF in olfactory ensheathing cells of rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(21):2290-2293. DOI：

摘要

目的观察Notch-1对嗅球成鞘细胞（olfactory ensheathing cells，OECs）神经生长因子（nerve growth factor，NGF）表达的影响。方法原代培养大鼠OECs，用有活性的Notch质粒（Notch

ICV

），无活性的Notch质粒（Notch

）以及空质粒分别转染OECs，将实验对象分成Notch

ICV

转染组，Notch

转染组和空白质粒组，应用免疫荧光双标、Western blot及RT-PCR技术，检测各组OECs中NGF及其mRNA的表达变化。结果转染Notch-1后，OECs中NGF的表达明显增高。免疫荧光双标结果显示，Notch

ICV

转染组OECs 中NGF的表达较Notch

转染组和空白质粒组均明显增高（P0.05）；RT-PCR结果显示，Notch

ICV

转染组OECs中NGF mRNA的表达（0.496 1±0.026 9）均高于Notch

转染组（0.244 5±0.013 4）及空白质粒组（0.245 9±0.016 0）（P0.01）；Western blot结果显示，Notch

ICV

转染组OECs中NGF的表达（0.887 5±0.016 6）均高于Notch

转染组（0.686 2±0.059 2）和空白质粒组（0.682 1±0.067 0）（P0.01）。结论 Notch-1转染OECs后，其NGF的表达上调，提示Notch-1能增强OECs中NGF的合成。

Abstract

Objective To observe the effect of Notch-1 on expression of nerve growth factor (NGF) in olfactory ensheathing cells (OEC) of rats. Methods Rats were divided into active Notch plasmid (Notch

ICV

) group

inactive Notch plasmid (Notch

) group

and empty plasmid group. Notch

ICV

Notch

and empty plasmid were transfected into OEC of rats. Immunofluorescence

Western blotting and RT-PCR were used respectively to detect the expression of NGF and NGF mRNA in OEC of rats. Results Immunofluorescence showed that the expression level of NGF was significantly higher in Notch

ICV

group than in Notch

group and empty plasmid group (P0.05). RT-PCR demonstrated that the expression level of NGF mRNA was significantly higher in Notch

ICV

group (0.496 1±0.026 9) than in Notch

group (0.244 5±0.013 4) and empty plasmid group (0.245 9±0.016 0) (P0.01). Western blotting displayed that the expression level of NGF was significantly higher in Notch

ICV

group (0.887 5±0.016 6) than in Notch

group (0.686 2±0.059 2) and empty plasmid group (0.682 1±0.067 0) (P0.01). Conclusion Expression of NGF is up-regulated in Notch-1 transfected OEC

and Notch-1 can increase the synthesis of NGF in OEC.

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