Effects of estradiol on apoptosis of mice endothelial progenitor cells and in vitro neovascularization

赵晓辉; 吴楠; 尹扬光; 方玉强; 宋明宝; 于世勇; 陈剑飞; 崔斌; 黄岚

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Effects of estradiol on apoptosis of mice endothelial progenitor cells and in vitro neovascularization

更新时间：2026-01-09

- Effects of estradiol on apoptosis of mice endothelial progenitor cells and in vitro neovascularization
- Journal of Army Medical University Vol. 32, Issue 13, Pages: 1399-1402(2010)
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  CLC： R322.12;R329.28;R977.12
- Published：2010
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赵晓辉,吴楠,尹扬光,方玉强,宋明宝,于世勇,陈剑飞,崔斌,黄岚.雌二醇调控小鼠内皮祖细胞凋亡及体外血管新生研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1399-1402.

　Zhao Xiaohui,Wu Nan,Yin Yangguang,Fang Yuqiang,Song Mingbao,Yu Shiyong,Chen Jianfei,Cui Bin,Huang Lan.Effects of estradiol on apoptosis of mice endothelial progenitor cells and in vitro neovascularization[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1399-1402.
赵晓辉,吴楠,尹扬光,方玉强,宋明宝,于世勇,陈剑飞,崔斌,黄岚.雌二醇调控小鼠内皮祖细胞凋亡及体外血管新生研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1399-1402. DOI：

　Zhao Xiaohui,Wu Nan,Yin Yangguang,Fang Yuqiang,Song Mingbao,Yu Shiyong,Chen Jianfei,Cui Bin,Huang Lan.Effects of estradiol on apoptosis of mice endothelial progenitor cells and in vitro neovascularization[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1399-1402. DOI：

摘要

目的观察雌二醇（estradiol，E

）对体外培养骨髓来源内皮祖细胞(endothlial progenitor cells，EPCs)分化及凋亡的影响。方法密度梯度离心法获取骨髓单个核细胞，流式细胞分析、FITC-荆豆凝集素Ⅰ、DiI-乙酰化低密度脂蛋白荧光双染鉴定。单个核细胞培养7 d后进行实验分为对照组、0.01 μmol/L E

组、0.1 μmol/L E

组、1 μmol/L E

组。分别采用流式细胞仪、体外管型生成实验观察EPCs的凋亡及体外血管新生的变化。结果雌二醇治疗组体外血管新生实验评分分别为0.01 μmol/L组（2.91±0.70）；0.1 μmol/L组（4.04±0.66）； 1 μmol/L组（4.73±0.48），较对照组（2.13±0.59）均明显增加（P0.01）；流式细胞仪检测过氧化氢诱导的雌二醇治疗组EPCs凋亡率分别0.1 μmol/L组[（0.24±0.01），(P0.05）

]

； 1 μmol/L组[（0.21±0.02），（P0.01）

]

，较对照组（0.29±0.02）明显降低；0.01 μmol/L雌二醇治疗后EPCs凋亡率无明显变化[（0.27±0.03），（P＞0.05）

]

。结论 E

可抑制EPCs凋亡、促进EPCs体外血管新生。

Abstract

Objective To observe the effect of estradiol (E

) on apoptosis of endothelial progenitor cells (EPC) and in vitro neovascularization. Methods Bone marrow mononuclear cells (MNC)

harvested by density gradient centrifugation

were double-stained with FITC-agglutinin 1 and DiI-acetylated low density lipoprotein fluorescence and analyzed by flow cytometry. Seven days after culture

MNC were divided into control group

0.01

μmol/L E

group

0.1 μmol/L group and 1 μmol/L E

group. After treated with E

at the concentrations of 0.01

0.1

and 1 μmol/L

apoptosis of EPC and in vitro neovascularization in experimental group were detected with a flow cytometer. Results E

used at the concentrations of 0.01

0.01

and 1 μmol/L

promoted in vitro tube formation in experimental group and control group (2.91±0.70

4.04±0.66 and 4.73±0.48 vs 2.13±0.59)

and apoptosis of EPC in experimental group decreased (0.24±0.01 and 0.21±0.02 vs 0.29±0.02 and 0.29±0.02

P0.01). Conclusion E

can inhibit apoptosis of EPC and promote in vitro neovascularization.

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