Effects of hyperlipidemia on expression of Foxp3 in ApoE-knockout mice

王治校; 王崇全; 何朝荣; 詹中群; 王俊峰; 李大主

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Effects of hyperlipidemia on expression of Foxp3 in ApoE-knockout mice

更新时间：2026-01-09

- Effects of hyperlipidemia on expression of Foxp3 in ApoE-knockout mice
- Journal of Army Medical University Vol. 32, Issue 13, Pages: 1395-1398(2010)
- 作者机构：
- 作者简介：
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- DOI：
  CLC： R541.4;R589.2;R394.3
- Published：2010
- 稿件说明：
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王治校,王崇全,何朝荣,詹中群,王俊峰,李大主.高血脂对小鼠Foxp3基因表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1395-1398.

　Wang Zhixiao,Wang Chongquan,He Chaorong,Zhan Zhongqun,Wang Junfeng,Li Dazhu.Effects of hyperlipidemia on expression of Foxp3 in ApoE-knockout mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1395-1398.
王治校,王崇全,何朝荣,詹中群,王俊峰,李大主.高血脂对小鼠Foxp3基因表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1395-1398. DOI：

　Wang Zhixiao,Wang Chongquan,He Chaorong,Zhan Zhongqun,Wang Junfeng,Li Dazhu.Effects of hyperlipidemia on expression of Foxp3 in ApoE-knockout mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1395-1398. DOI：

摘要

目的研究高血脂对小鼠Foxp3基因表达的影响，并观察ApoE

-/-

小鼠动脉粥样硬化中Foxp3的表达及鉴定其功能。方法选取8周龄雄性C57BL/6J ApoE

-/-

及其同系野生型小鼠16只，分为对照组和高脂饮食组(n=8)，构建小鼠动脉粥样硬化模型，比较不同组小鼠血脂水平、斑块面积和不同器官组织中Foxp3

CD4

CD25

Tregs细胞的数目；利用RT-PCR和Western blot分析组织中Foxp3基因转录水平和Foxp3蛋白表达水平。结果与对照组[TG(2.0±0.1)mmol/L、TC(20.6±0.1) mmol/L

]

比较，高脂饮食组小鼠血脂水平[TG(2.8±0.2)mmol/L、TC(28.2±1.2) mmol/L

]

明显升高（P0.01），斑块面积(25 044.32±4 920.42)μm

显著增加（P0.01），而不同器官组织中Foxp3

CD4

CD25

Tregs细胞的数目显著减少(P0.05)，Foxp3基因转录水平(P0.01)和Foxp3蛋白表达(P0.05)降低。结论高脂能抑制不同免疫器官Foxp3基因的表达和功能，可能是高脂致动脉粥样硬化的机制之一。

Abstract

Objective To examine the effect of hyperlipemia on expression of Foxp3 gene in ApoE

-/-

mice. Methods Sixteen male ApoE

-/-

mice were randomly divided into control group and high fat diet group

8 in each group. Blood-lipid levels

plaque areas

and the number of Foxp3

CD4

CD25

regulatory T cells in different organ tissues were compared between the 2 groups. Transcription and expression levels of Foxp3 gene in tissues of

mice were detected by RT-PCR and Western blotting

respectively. Results The blood-lipid level (2.8±0.2 mmol/L for TG

28.2±1.2 mmol/L for TC) was significantly higher

the plaque area was remarkably larger (25 044.32±4 920.42 μm

)

the number of Foxp3

CD4

CD25

regulatory T cells was significantly lesser

and the transcription and expression levels of Foxp3 gene were notably lower in different organ tissues

in high fat diet group than in control group (P0.05). Conclusion Hyperlipemia can inhibit the expression and function of Foxp3 gene in different immune organs

which may be one of the mechanisms underlying atherosclerosis due to hyperlipemia.

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