β-catenin decreases hS100A6-induced inhibition in osteosarcoma cell lines MG63 and U2OS

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β-catenin decreases hS100A6-induced inhibition in osteosarcoma cell lines MG63 and U2OS

更新时间：2026-01-09

- β-catenin decreases hS100A6-induced inhibition in osteosarcoma cell lines MG63 and U2OS
- Journal of Army Medical University Vol. 32, Issue 16, Pages: 1703-1707(2010)
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  CLC： R738.1;R394.3;R73-362
- Published：2010
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卫佳,李星星,陈英华,吴丽美,马闻,何通川,周兰.β-catenin降低外源性hS100A6对骨肉瘤细胞的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(16):1703-1707.

　Wei Jia,Li Xingxing,Chen Yinghua,Wu Limei,Ma Wen,He Tongchuan,Zhou Lan.β-catenin decreases hS100A6-induced inhibition in osteosarcoma cell lines MG63 and U2OS[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(16):1703-1707.
卫佳,李星星,陈英华,吴丽美,马闻,何通川,周兰.β-catenin降低外源性hS100A6对骨肉瘤细胞的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(16):1703-1707. DOI：

　Wei Jia,Li Xingxing,Chen Yinghua,Wu Limei,Ma Wen,He Tongchuan,Zhou Lan.β-catenin decreases hS100A6-induced inhibition in osteosarcoma cell lines MG63 and U2OS[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(16):1703-1707. DOI：

摘要

目的研究外源性hS100A6抑制骨肉瘤细胞作用与Wnt/β-catenin信号途径的关系。方法用携带人β-catenin 及其siRNA基因的重组腺病毒Adβ-catenin和AdSiβ-catenin分别上调和下调MG63和U2OS细胞中β-catenin水平，再用重组hS100A6处理这些细胞，然后通过MTT法检测细胞增殖能力的变化，Hochest染色法检测细胞凋亡的变化，划痕愈合实验和Transwell实验检测细胞迁移能力的变化。结果 ①外源性hS100A6抑制2株骨肉瘤细胞的增殖（P0.05）；β-catenin过表达和低表达则分别减弱和增强hS100A6对这2株细胞的增殖抑制作用（P0.05）；②外源性hS100A6促进2株骨肉瘤细胞的凋亡（P0.05）；β-catenin过表达和低表达则分别增强和减弱hS100A6对这2株细胞的促凋亡作用（P0.05）；③外源性hS100A6抑制骨肉瘤细胞株MG63的迁移（P0.05）；β-catenin过表达和低表达则分别减弱和增强hS100A6对MG63的迁移抑制作用（P0.05）。结论 β-catenin负性调节hS100A6对骨肉瘤的抑制作用；hS100A6对骨肉瘤的抑制作用可能经由Wnt信号途径及其他信号途径共同调控。

Abstract

Objective To investigate the relationship between the inhibition of hS100A6 on osteosarcoma cell lines MG63 and U2OS and Wnt/ β - catenin signaling pathway. Methods Adenoviruses carrying human β - catenin and its siRNA gene respectively were used to up - regulate and down - regulate the expression of β- catenin in MG63 and U2OS cells. Then MTT assay

Hoechst staining

scratching test and Transwell assay were used to detect cells proliferation

apoptosis and migration

respectively after the treatment of hS10

0A6. Results Ad β - catenin and AdSi β - catenin up - and down - regulated β - catenin expression in MG63 and U2OS (

P

0.05)

respectively. Exogenous hS100A6 inhibited the proliferation of wild osteosarcoma cell lines MG63 and U2OS (

P

0 . 05)

while over - or low - expression of β - catenin reduced or enhanced the inhibitive effect of S100A6 on cell proliferation respectively (

P

0 . 05). Exogenous hS100A6 promoted the apoptosis of 2 wild cell lines (

P

0 . 05)

but over- or low-expression of β - catenin enhanced or reduced the ability of hS100A6 respectively (

P

0 . 05). Exogenous hS100A6 inhibited the migration of MG63 cells

however

over- or low-expression of β - catenin reduced or enhanced the ability of hS100A6 to inhibit migration (

P

0 . 05). Conclusion The inhibitory role of hS100A6 on osteosarcoma cells is regulated negatively by β - atenin. This inhibitory role may be controlled by Wnt/ β- atenin and other signaling pathways.

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