Role of aquaporin 4 in swollen rat astrocytes after oxygen-glucose deprivation

唐兆华; 廖正步; 蒋光元; 支兴刚; 石全红; 何朝晖; 詹彦

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Role of aquaporin 4 in swollen rat astrocytes after oxygen-glucose deprivation

更新时间：2026-01-09

- Role of aquaporin 4 in swollen rat astrocytes after oxygen-glucose deprivation
- Journal of Army Medical University Vol. 32, Issue 13, Pages: 1387-1390(2010)
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  CLC： R322.8;R329.27;R362
- Published：2010
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唐兆华,廖正步,蒋光元,支兴刚,石全红,何朝晖,詹彦.水通道蛋白4在缺氧缺糖后水肿星形胶质细胞中的作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1387-1390.

　Tang Zhaohua,Liao Zhengbu,Jiang Guangyuan,Zhi Xinggang,Shi Quanhong,He Zhaohui,Zhan Yan.Role of aquaporin 4 in swollen rat astrocytes after oxygen-glucose deprivation[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1387-1390.
唐兆华,廖正步,蒋光元,支兴刚,石全红,何朝晖,詹彦.水通道蛋白4在缺氧缺糖后水肿星形胶质细胞中的作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1387-1390. DOI：

　Tang Zhaohua,Liao Zhengbu,Jiang Guangyuan,Zhi Xinggang,Shi Quanhong,He Zhaohui,Zhan Yan.Role of aquaporin 4 in swollen rat astrocytes after oxygen-glucose deprivation[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1387-1390. DOI：

摘要

目的探讨水通道蛋白4（aquaporin 4，AQP4）在缺氧缺糖培养后水肿星形胶质细胞中的表达变化与作用。方法星形胶质细胞分为正常组和缺氧缺糖组，缺氧缺糖组细胞经过5 h缺氧缺糖后恢复正常条件培养至0.5、2、8、24 h和48 h 5个时间点时

用光镜观察细胞形态变化，RT-PCR及免疫荧光法测定AQP4的mRNA及蛋白的表达变化。结果 ①在缺氧缺糖后0.5 h，细胞肿胀逐渐形成并继续加重，2 h肿胀达到高峰，至8 h和24 h时肿胀逐渐消退，48 h细胞基本恢复正常形态。②RT-PCR和免疫荧光检测：在缺氧缺糖后0.5 h，AQP4 mRNA和蛋白的表达均较正常组明显下降（P0.05），2 h回升，8 h明显超过正常水平，达最高值（P0.05），24 h有所下降，但仍明显高于正常水平（P0.05），至48 h时mRNA和蛋白的表达均基本恢复正常水平。结论缺氧缺糖后星形胶质细胞水肿形成及消退期AQP4的不同表达变化可能都是细胞的一种自身保护机制，从而尽量维持细胞的正常形态和功能。

Abstract

Objective To study the expression of aquaporin 4（AQP4）in swollen astrocytes after oxygen-glucose deprivation (OGD). Methods Astrocytes primarily cultured from 2-day-old SD rats were divided into normal group and OGD group. Five hours after OGD

the cells were cultured for 0.5

24 and 48 h in normal culture medium

respectively. Morphological alterations of astrocytes were observed under a light microscope. Expression of AQP4 mRNA and protein in astrocytes was detected by RT-PCR and immunofluorescence assay

respectively. Results Swelling of astrocytes occurred in 0.5 h after OGD

reached its peak in 2 h after OGD

gradually disappeared in 8-24 h after OGD

and became almost normal in 48 h after OGD. RT-PCR and immunofluorescence assay showed that the expression level of AQP4 mRNA and protein in astrocytes began to decrease

increase and return to normal

respectively

in 0.5

and 48 h after OGD. Conclusion The expression level of AQP4 is decreased in swollen astrocytes in 5 h after OGD possibly by preventing the entry of it-mediated water into astrocytes

and is increased during the recovery stage of cell swelling by promoting excretion of water from swollen astrocytes.

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