D<sub>4</sub> dopamine receptor suppresses AngⅡ-mediated proliferation of vascular smooth muscle cells in vitro

程江华; 杨剑; 于长青; 韩愈; 何多芬; 王旭开; 王红勇; 杨成明; 曾春雨

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D₄ dopamine receptor suppresses AngⅡ-mediated proliferation of vascular smooth muscle cells in vitro

更新时间：2026-01-09

- D₄ dopamine receptor suppresses AngⅡ-mediated proliferation of vascular smooth muscle cells in vitro
- Journal of Army Medical University Vol. 32, Issue 20, Pages: 2168-2171(2010)
- 作者机构：
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  CLC： R322.12;R329.28;R544.102
- Published：2010
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程江华,杨剑,于长青,韩愈,何多芬,王旭开,王红勇,杨成明,曾春雨.多巴胺D4受体对血管紧张素Ⅱ介导的血管平滑肌细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(20):2168-2171.

　Cheng Jianghua,Yang Jian,Yu Changqing,Han Yu,He Duofen,Wang Xukai,Wang Hongyong,Yang Chengming,Zeng Chunyu.D4 dopamine receptor suppresses AngⅡ-mediated proliferation of vascular smooth muscle cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(20):2168-2171.
程江华,杨剑,于长青,韩愈,何多芬,王旭开,王红勇,杨成明,曾春雨.多巴胺D4受体对血管紧张素Ⅱ介导的血管平滑肌细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(20):2168-2171. DOI：

　Cheng Jianghua,Yang Jian,Yu Changqing,Han Yu,He Duofen,Wang Xukai,Wang Hongyong,Yang Chengming,Zeng Chunyu.D4 dopamine receptor suppresses AngⅡ-mediated proliferation of vascular smooth muscle cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(20):2168-2171. DOI：

摘要

目的观察多巴胺D

受体对血管紧张素Ⅱ（angiotensin Ⅱ

AngⅡ）介导的血管平滑肌细胞(vascular smooth muscle cells

VSMCs)增殖的影响。方法以大鼠胸主动脉平滑肌细胞株（A10细胞）、原代的Wistar-Kyoto (WKY VSMCs）和自发性高血压大鼠(SHR)胸主动脉血管平滑细胞（VSMCs）为研究对象，观察刺激D

受体对AngⅡ促增殖作用的影响以及D

受体特异性阻断剂L745870对该作用的阻断效应。细胞增殖采用MTT和细胞计数方法测定。结果 AngⅡ(10

-7

mol/L)可促进A10细胞增殖，最大增殖幅度为64%。D

受体激动剂PD168077本身对A10细胞无增殖影响，但D

受体特异激动剂PD168077可抑制AngⅡ(10

-7

mol/L) 介导的血管平滑肌细胞的增殖作用，最大抑制幅度为40%；应用D

受体阻断剂L745870后该抑制作用丧失；D

受体对AngⅡ介导血管增殖的作用在SHR和WKY大鼠的VSMCs之间并无区别。结论 D

受体对AngⅡ介导的血管平滑肌细胞增殖具有抑制作用，该作用可能在一定程度上抑制了高血压病所伴发的血管平滑肌细胞增殖。

Abstract

Objective To investigate the effect of D

dopamine receptor on AngiotensinⅡ(AngⅡ) mediated proliferation of vascular smooth muscle cells (VSMCs). Methods A10 cells (rat aortic vascular smooth muscle cells)

primary WKY (WistarKyoto) and SHR(spontaneously hypertensive rat) VSMCs were used in this study under the concentration of AngⅡ from 10

-10

to 10

-6

mol/L in

presence or absence of D

dopamine receptor agonist PD168077 (10

-10

to 10

-6

mol/L) or its blocker L745870 (10

-7

mol/L). The proliferation of VSMC cells was determined by MTT assay and cell counting. Results AngⅡ (10

-7

mol/L) enhanced proliferation of A10 cells (maximal proliferative amplitude 64%). D

receptor

by itself

had no effect on cell proliferation

but blocked the AngⅡ (10

-7

mol/L)mediated proliferation of A10 cells (maximal inhibitory effect 40%)

while the effect was inhibited in the presence of D

receptor blocker L745870. The above-mentioned effect of D

receptor on AngⅡ-mediated proliferation had no difference between SHR and WKY VSMCs. Conclusion Activation of D

receptor inhibits AngⅡ-mediated proliferation of vascular smooth muscle cells

which might be helpful to alleviate the proliferation of VSMCs in essential hypertension.

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D4 dopamine receptor suppresses AngⅡ-mediated proliferation of vascular smooth muscle cells in vitro

DOI：

摘要

Abstract

关键词

Keywords

references

D₄ dopamine receptor suppresses AngⅡ-mediated proliferation of vascular smooth muscle cells in vitro