Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice

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Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice

更新时间：2026-01-09

- Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice
- Journal of Army Medical University Vol. 32, Issue 13, Pages: 1383-1386(2010)
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  CLC： R394.2
- Published：2010
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张政,罗勇军,彭惠民,何晓燕.早期糖尿病肾病相关微小RNA在小鼠肾脏的表达变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1383-1386.

　Zhang Zheng,Luo Yongjun,Peng Huimin,He Xiaoyan.Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1383-1386.
张政,罗勇军,彭惠民,何晓燕.早期糖尿病肾病相关微小RNA在小鼠肾脏的表达变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1383-1386. DOI：

　Zhang Zheng,Luo Yongjun,Peng Huimin,He Xiaoyan.Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(13):1383-1386. DOI：

摘要

目的建立db/db小鼠早期糖尿病肾病（diabetic nephropathy，DN）微小RNA（microRNA，miRNA）的差异表达谱。方法采用12只9周龄高血糖且24 h尿白蛋白显著增高的db/db小鼠作为早期糖尿病肾病组，12只9周龄db/m小鼠作为正常对照组，用于miRNA芯片和实时荧光定量RT-PCR检测。Trizol法抽提肾脏总RNA，YM-100微离心滤器富集小RNA，芯片技术检测DN组和对照组miRNAs的差异表达谱。应用实时荧光定量RT-PCR验证部分差异miRNAs，运用生物信息学技术预测部分相关miRNAs的靶基因。结果芯片结果显示，66个miRNA在DN组和对照组存在差异表达（P0.01），其中35个miRNAs在DN组呈高表达，31个miRNAs在DN组呈低表达，差异倍数从1.08~10.20。实时荧光定量RT-PCR进一步验证部分miRNA的表达，结果显示3个miRNAs在DN组表达上调，分别为：miR-196a、miR-98和miR-29c；4个表达在DN组下调，分别为miR-21、miR-451、miR-709和miR-187，差异显著（P0.01）。结论部分miRNAs在DN和正常对照肾脏组织中存在差异表达，可能参与了DN发生的分子机制。

Abstract

Objective To establish the differential expression profile of early diabetic nephropathy (DN)- related microRNA (miRNA) in kidneys of db/db mice. Methods Twelve 9-week-old male db/db mice with hyperglycemia and significantly elevated urinary albumin excretion were used as an early DN group，and another twelve 9-week-old male db/m mice were used as a control group. Total mRNA was extracted from kidney tissues of mice and miRNA was concentrated with the YM-100 micro-centrifugation filter. Differential expression profile of miRNA in DN and control groups was detected by miRNA assay and RT-PCR

respectively. Target gene of related miRNA was detected with bioinformatics technology. Results miRNA assay showed that 66 miRNAs were differentially expressed in DN and control groups (P0.01). Of the 66 miRNAs

35 and 31 were highly and lowly expressed

respectively

in DN group

with a difference of 1.08 to 10.20 folds. RT-PCR showed that the expression of 3 miRNAs was up-regulated (miR-196a

miR-98 and miR-29c) and the expression of 4 miRNAs was down-regulated (miR-21

miR-451

miR-709 and miR-187) in DN group（P0.01）. Conclusion Some miRNAs are differentially expressed in the kidney tissues of DN and normal mice

suggesting that miRNAs may be involved in the molecular mechanism of DN.

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