Objective    To construct a yeast expression vector containing human TIP60β gene (a spliced form of HIV-1 tat interactive protein

HTATIP

60×10

3

) and screen its interaction proteins by yeast two-hybrid.     Methods    Human TIP60β gene fragment was amplified by RT-PCR and cloned into pGBKT7 vector. Using TIP60β as bait

the proteins interacting with TIP60β were screened from a human liver cDNA library. The positive clones were analyzed by bioinformatic methods.     Results    Human TIP60β cDNA was successfully amplified and cloned into the pGBKT7 vector and indentified by double enzyme digestion and DNA sequencing. Using yeast two-hybrid

32 clones were screened fro

m a human liver cDNA library

and 9 positive clones including histone deacetylase 7A (HDAC7A)

mouse double minute 2 (DMD2)

B-cell CLL/lymphoma 3 (BCL3)

endothelin receptor type A (EDNRA)

androgen receptor (AR)

PHD finger protein 17 (PHF17)

Ataxia Telangiectasia Mutated (ATM)

CAMP responsive element binding protein 1 (CREB1)

orphan steroid receptor BD73 were verified after sequencing.      Conclusion    The screened interaction proteins with TIP60β by yeast two-hybrid may be involved in the roles of transcriptional regulation of TIP60β.