Effect of thymosin α1 on percentage of T-lymphocyte subsets in in vitro peripheral blood mononuclear cells from gastric cancer patients

杨霞; 钱锋; 刘凯军; 何海洋; 兰远志; 田易; 傅晓岚; 李健; 张记; 申子刚; 李晋涛; 吴玉章

您当前的位置：

首页 >

文章列表页 >

Effect of thymosin α1 on percentage of T-lymphocyte subsets in in vitro peripheral blood mononuclear cells from gastric cancer patients

更新时间：2026-01-09

- Effect of thymosin α1 on percentage of T-lymphocyte subsets in in vitro peripheral blood mononuclear cells from gastric cancer patients
- Journal of Army Medical University Vol. 32, Issue 12, Pages: 1249-1252(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.12;R735.2;R977.6
- Published：2010
- 稿件说明：
移动端阅览
杨霞,钱锋,刘凯军,何海洋,兰远志,田易,傅晓岚,李健,张记,申子刚,李晋涛,吴玉章.胸腺肽α1体外刺激胃癌患者外周血单个核细胞对淋巴细胞亚群影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1249-1252.

　Yang Xia,Qian Feng,Liu Kaijun,He Haiyang,Lan Yuanzhi,Tian Yi,Fu Xiaolan,Li Jian,Zhang Ji,Shen Zigang,Li Jintao,Wu Yuzhang.Effect of thymosin α1 on percentage of T-lymphocyte subsets in in vitro peripheral blood mononuclear cells from gastric cancer patients[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1249-1252.
杨霞,钱锋,刘凯军,何海洋,兰远志,田易,傅晓岚,李健,张记,申子刚,李晋涛,吴玉章.胸腺肽α1体外刺激胃癌患者外周血单个核细胞对淋巴细胞亚群影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(12):1249-1252. DOI：

　Yang Xia,Qian Feng,Liu Kaijun,He Haiyang,Lan Yuanzhi,Tian Yi,Fu Xiaolan,Li Jian,Zhang Ji,Shen Zigang,Li Jintao,Wu Yuzhang.Effect of thymosin α1 on percentage of T-lymphocyte subsets in in vitro peripheral blood mononuclear cells from gastric cancer patients[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(12):1249-1252. DOI：

摘要

目的观察胸腺肽α1（thymosin α1，Tα1）体外刺激培养胃癌患者外周血单个核细胞（peripheral blood mononuclear cells，PBMCs）对淋巴细胞亚群分化及分泌细胞因子谱的影响，分析Tα1对肿瘤患者免疫功能的影响。方法分离32例胃癌患者及18例健康自愿者PBMCs，50、10、1 μg/ml Tα1体外刺激培养2 d；流式细胞仪检测CD4

、CD8

、CD4

CD25

Foxp3

T细胞百分比、CD4

/CD8

变化及分泌Th1/Th2细胞因子谱变化。结果 Tα1体外刺激PBMCs后，胃癌患者及健康自愿者的CD4

、CD8

T细胞水平及CD4

/CD8

比值均无明显变化；健康自愿者CD4

CD25

Foxp3

T细胞水平无明显变化，胃癌患者CD4

CD25

Foxp3

T细胞水平明显提高（P0.05）； 1 μg/ml Tα1促进胃癌患者PBMCs分泌IL-1β、TNF-α，10 μg/ml促进胃癌患者PBMCs分泌IL-6(P0.05)，健康自愿者PBMCs分泌细胞因子谱变化不明显（P0.05）。结论 Tα1体外刺激胃癌患者PBMCs，提高CD4

CD25

Foxp3

Treg细胞亚群水平，可能对肿瘤患者抗肿瘤免疫起抑制效应。

Abstract

Objective To investigate the effect of thymosin α1 (Tα1) on cellular immune function in gastric cancer patients through observing its treatment on the differentiation of T-lymphocyte subsets from screened peripheral blood mononuclear ce

lls (PBMCs). Methods PBMCs were obtained by centrifugation of blood samples from 18 healthy subjects and 32 patients with gastric cancer

and then cultured in the presence of culture medium with addition of Tα1 at 50

10 and 1 μg/ml for 2 d. T lymphocyte subsets (such as CD4

CD8

and CD4

CD25

Foxp3

T cells) and Th1/Th2 multiplex cytokines were detected by flow cytometry (FCM). Results After PBMCs isolated from healthy people and patients were incubated with or without Tα1

there was no significant change in percentage of CD4

CD8

peripheral lymphocyte subsets and ratio of CD4

/CD8

. There was no obvious change in the percentage of CD4

CD25

Foxp3

T lymphocyte subsets in the normal control

but a significant increase was observed in the cells from patients with gastric cancer after treatment (P0.05). Tα1 at 1 μg/ml induced the secretions of IL-1β and TNF-α in PBMCs from patients with respect to PBS treated cells

and 10 μg/ml of Tα1 increased the secretion of IL-6 in patient PBMCs. However

Tα1 had no such effect on multiplex cytokines in PBMCs from healthy people. Conclusion Tα1 increases the percentage of CD4

CD25

Foxp3

Treg cells in PBMCs from gastric cancer patients

and it may increase risk of patients for tumor immunotolerance.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Th1/Th2 cytokines’ profile in peripheral blood and synovial fluid of patients with rheumatoid arthritis

Accessible chromatin upregulates circadian rhythm transcription factor NPAS2 to promote Ferroptosis Resistance in Gastric Cancer Cells

Clinical characterization and prognostic modeling of second primary malignancies following gastric adenocarcinoma: a SEER database-based study

Hypoxia specifically induces CADM3 expression in pulmonary endothelial cells and promotes monocyte adhesion

Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca2+-mediated autophagy

Related Author

李景怡

方勇飞

邹丽云

张晋宇

傅晓岚

柏干苹

万瑛

吴玉章

Related Institution

School of Medicine， Chongqing University

Jinfeng Laboratory

Southwest University Medical Research Institute

College of Pharmaceutical Sciences， Southwest University

Department of Gastroenterology， Chongqing Key Laboratory of Precise Prevention and Treatment of Digestive Malignancies， Daping Hospital， Army Medical University

⁰