Regulatory role of interleukin-1β in vascular calcium sensitivity and its relationship with PKC and Rho kinase in rabbits following endotoxic shock

梁家林; 李涛; 杨光明; 徐竞; 贺教江; 陈垦; 刘良明

您当前的位置：

首页 >

文章列表页 >

Regulatory role of interleukin-1β in vascular calcium sensitivity and its relationship with PKC and Rho kinase in rabbits following endotoxic shock

更新时间：2026-01-09

- Regulatory role of interleukin-1β in vascular calcium sensitivity and its relationship with PKC and Rho kinase in rabbits following endotoxic shock
- Journal of Army Medical University Vol. 32, Issue 20, Pages: 2158-2162(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R631.102;R322.12;R392.11
- Published：2010
- 稿件说明：
移动端阅览
梁家林,李涛,杨光明,徐竞,贺教江,陈垦,刘良明.白介素-1β对家兔内毒素休克血管钙敏感性的调节作用及与Rho激酶和PKC的关系[J].陆军军医大学学报(原第三军医大学学报),2010,32(20):2158-2162.

　Liang Jialin,Li Tao,Yang Guangming,Xu Jing,He Jiaojiang,Chen Ken,Liu Liangming.Regulatory role of interleukin-1β in vascular calcium sensitivity and its relationship with PKC and Rho kinase in rabbits following endotoxic shock[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(20):2158-2162.
梁家林,李涛,杨光明,徐竞,贺教江,陈垦,刘良明.白介素-1β对家兔内毒素休克血管钙敏感性的调节作用及与Rho激酶和PKC的关系[J].陆军军医大学学报(原第三军医大学学报),2010,32(20):2158-2162. DOI：

　Liang Jialin,Li Tao,Yang Guangming,Xu Jing,He Jiaojiang,Chen Ken,Liu Liangming.Regulatory role of interleukin-1β in vascular calcium sensitivity and its relationship with PKC and Rho kinase in rabbits following endotoxic shock[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(20):2158-2162. DOI：

摘要

目的观察白介素-1β（IL-1β）对家兔内毒素休克及离体血管钙敏感性影响及与PKC、Rho激酶的关系。方法健康成年家兔35只，雌雄不拘，完全随机分为正常对照组，LPS 30 min组，LPS 1 h组，LPS 2 h组，LPS 4 h组，每组7只，经耳缘静脉注射1 mg/kg LPS（O

111

∶B

）复制家兔内毒素休克模型，在给LPS后30 min、1、2、4 h取血清及肠系膜上动脉（superior mesenteric artery， SMA），用ELISA方法检测不同时相点血清中IL-1β的浓度；用离体血管环张力测定技术，测血管环钙敏感性的变化，分析其与血清IL-1β浓度的相关关系。另取家兔70只，采用随机数字表分为正常对照组，25 ng/ml IL-1β组，50 ng/ml IL-1β组，100 ng/ml IL-1β组， 150 ng/ml IL-1β组，200 ng/ml IL-1β组，200 ng/ml IL-1β+PMA组，200 ng/ml IL-1β+staurosporine组，200 ng/ml IL-1β+AngⅡ组，200 ng/ml IL-1β+Y27632组，每组7只。无菌条件下取正常家兔SMA，检测不同浓度重组人IL-1β对其钙敏感性的影响及与PKC、Rho激酶的关系。结果给LPS后30 min至1 h内成功复制出内毒素休克模型，测其平均动脉压（mean artery pressure

MAP）下降30%以上，血清中IL-1β在2 h后即开始上升，至4 h时显著升高（P0.01）；SMA钙敏感性在给LPS后30 min、1 h较正常对照组明显增高（P0.01），而在给LPS后2、4 h则明显降低（P0.01）

其变化趋势与血清IL-1β水平变化呈显著负相关（r=-0.675，P0.01）。IL-1β可诱导离体SMA钙敏感性下降，其作用可被PKC和Rho激酶的激动剂拮抗。结论 IL-1β能够降低血管钙敏感性，且该作用与PKC、Rho激酶密切相关，IL-1β对内毒素休克后血管钙失敏可能有重要介导作用。

Abstract

Objective To investigate the effect of interleukin-1β (IL-1β) on vascular calcium se

nsitivity in vitro and in rabbit after endotoxic shock

and to evaluate whether the effect is concerned with protein kinase C (PKC) and Rho kinase. Methods A total of 35 adult rabbits were divided into 5 groups: normal control

LPS 30minute

LPS 1hour

LPS 2hour

and LPS 4hour groups (n=7). Rabbits were given 1 mg/kg of LPS (O

111

∶B

) via ear-border vein to induce endotoxic shock. Then blood serum and superior mesenteric artery (SMA) were collected to measure IL-1β concentration and calcium sensitivity respectively at correspording time points. The vascular calcium sensitivity was measured with an isolated organ perfusion system. Then the correlation of calcium sensitivity of SMAs with IL-1β concentration in blood serum was analyzed. Effects of different concentrations of IL-1β (25

100

150 and 200 ng/ml) on vascular calcium sensitivity of SMAs and the relationship to PKC and Rho kinase were also observed with the aid of their agonists

inhibitors and antagonists (200 ng/ml IL-1β+ 10

-7

mol/L PMA

+10

-7

/10

-6

/10

-5

mol/L staurosporine

+10

-5

mol/L Y27632

and +10

-9

mol/L AngⅡ). Results Endotoxic shock model was successfully produced in 30 min to 1 h after LPS injection. The IL-1β concentration of blood serum started to increase at 2 h after injection

and it was then elevated very obviously at 4 h after injection (P0.01). As compared to the control group

the calcium sensitivity of SMAs at early period (30 min and 1 h) after LPS injection was increased significantly (P0.01)

however

in 2 h and 4 h after LPS injection

it was decreased obviously (P0.01). The changes of vascular calcium sensitivity was positively correlated with the IL-1β concentration in blood serum (r=-0.675

P0.05). IL-1β reduced the calcium sensitivity of SMA in vitro

but the effect was reversed by PKC and Rho kinase agonists. Conclusion Our experiments reveal that IL-1β could decrease calcium sensitivity of rabbit in vitro

and its effect

is closely related to PKC and Rho kinase. IL-1β may play a very important role in calcium desensitization following endotoxic shock in rabbits.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

IL-1β regulates calcium sensitivity in superior mesenteric artery by changing PKC and Rho kinase activity in rabbits with endotoxic shock

Effect of TNF-α on calcium sensitivity in rabbits with endotoxin-induced shock and its relation with Rho-kinase and protein kinase C

Related Author

梁家林

贺教江

臧家涛

朱娱

刘良明

李涛

杨光明

徐竞

Related Institution

No data

⁰