Objective    To study the change of myelin sheath in adult rats having persistent epilepsy at their young age and then progressing to spontaneous recurrent epileptic seizure (SRS) in adult age.     Methods    A persistent epilepsy model of infantile rat was induced by intra-peritoneal injection with lithium chloride pilocarpine. Abnormal waves of epilepsy on electroencephalogram (EEG) were displayed with a multi-channel physiological signal collection system. Change of neurons in the hippocampus was observed with Nissl staining. Change of myelin sheath was observed with luxol fast blue (LFB) staining. Expression of myelin basic protein (MBP) was detected with immunohistochemical staining.      Results    More sharp

spike and ware

sharp and ware waves of epilepsy were observed on EEG of SRS model group than on EEG of normal group. The number of neurons stained with Nissl dye in the hippocampal CA1

CA3 and cingulated gyrus was decreased

with some cells degenerated and necrotized. The LFB stained area of corpus callosum and cingulum was significantly smaller in model group than in control group (P0.05). The average optical density of immune positive MBP deposit in primary somatosensory cortex barrel field (S1BF) and primary auditory cortex (Au1) field was significantly lower in model group than in control group(P0.05).      Conclusion    Chronic epilepsy attack in adult rats with persistent epilepsy at their young age may lead to demyelination in the central nervous system in adult age.