Transfection of wild type hepaCAM gene inhibits cell growth and VEGF expression in BIU-87 cells

杨淑哲; 罗春丽; 吴小候; 潘翠翠; 蔡晓钟

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Transfection of wild type hepaCAM gene inhibits cell growth and VEGF expression in BIU-87 cells

更新时间：2026-01-09

- Transfection of wild type hepaCAM gene inhibits cell growth and VEGF expression in BIU-87 cells
- Journal of Army Medical University Vol. 32, Issue 5, Pages: 490-493(2010)
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  CLC： R737.14;R394.2;R730.23
- Published：2010
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杨淑哲,罗春丽,吴小候,潘翠翠,蔡晓钟.HepaCAM基因转染对膀胱癌BIU-87细胞株生长及血管内皮生长因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(05):490-493.

　Yang Shuzhe,Luo Chunli,Wu Xiaohou,Pan Cuicui,Cai Xiaozhong.Transfection of wild type hepaCAM gene inhibits cell growth and VEGF expression in BIU-87 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(05):490-493.
杨淑哲,罗春丽,吴小候,潘翠翠,蔡晓钟.HepaCAM基因转染对膀胱癌BIU-87细胞株生长及血管内皮生长因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(05):490-493. DOI：

　Yang Shuzhe,Luo Chunli,Wu Xiaohou,Pan Cuicui,Cai Xiaozhong.Transfection of wild type hepaCAM gene inhibits cell growth and VEGF expression in BIU-87 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(05):490-493. DOI：

摘要

目的探讨外源性肝细胞黏附分子(hepatocyte cell adhesion molecule，hepaCAM)转染对人膀胱癌BIU87细胞株生长及血管内皮生长因子（vascular endothelial growth factors

VEGF）表达的影响。方法使用脂质体2000转染法将携带野生型hepaCAM基因的质粒（pEGFPN2-hepaCAM）及空载质粒（pEGFPN2）转染人膀胱癌BIU-87细胞，建立稳定过表达hepaCAM基因的BIU-87细胞株。以转染空载质粒及未转染的BIU-87细胞株作为对照，采用MTT法检测各组细胞生长能力，逆转录聚合酶链反应（RT-PCR）检测hepaCAM、VEGF mRNA水平的变化，Western blot检测VEGF蛋白水平的变化。结果 MTT实验示：hepaCAM基因转染（实验组）BIU-87细胞生长与空载体组和未处理组细胞比较明显受到抑制(P0.05)；hepaCAM基因在膀胱癌BIU-87细胞不表达，转染后hepaCAM mRNA明显增强（P0.05）；实验组VEGF mRNA表达（0.527±0.031）与未处理组（0.803±0.042）、空载组（0.750±0.040）比较明显下降（P0.05）。Western blot结果示：实验组BIU-87细胞VEGF蛋白表达（0.057±0.015）与未处理组（0.263±0.032）和空载组（0.280±0.036）比较明显下降（P0.05）。结论转染野生型hepaCAM基因可明显抑制膀胱癌BIU-87细胞的生长能力，并下调VEGF基因及蛋白的表达， hepaCAM基因可能是影响肿瘤的生长及侵袭转移的重要原因之一。

Abstract

Objective To investigate the effects of transfection of wild type hepatocyte cell adhesion molecule (hepaCAM) gene on the growth and metastasis of bladder cancer BIU-87 cells. Methods Plasmids pEGFPN2-hepaCAM and pEGFPN2 were respectively transfected into BIU-87 cells with Lipofectamine2000 to establish BIU-87 cells with stable over-expression of hepaCAM. MTT assay was used to observe the proliferation of hepaCAM transfection cells

pEGFPN2 transfection cells and untransfected cells. The mRNA expressions of hepaCAM and VEGF before and after the stable transfection were detected by reverse transcriptase polymerase chain reaction (RT-PCR). The protein level of VEGF in the above cell groups was analyzed by Western blot analysis. Results MTT assay showed that the proliferation of BIU-87 cells transfected with wild type of hepaCAM was markedly inhibited compared with that of control groups (P0.05). There was no expression of hepaCAM mRNA in BIU-87 cells

but the expression of hepaCAM mRNA of BIU-87 cells transfected with hepaCAM gene was markedly increased (P0.05). The expression of VEGF mRNA (0.527±0.031) in BIU-87 cells transfected with hepaCAM was markedly inhibited as compared with that of transfected with empty vector cells (0.750±0.040) and untransfected cells (0.803±0.042

P0.05). The results of Western blotting showed the protein expression of VEGF in BIU-87 cells transfected with hepaCAM (0.057±0.015) was markedly inhibited as compared with transfected with empty vector cells (0.280±0.036) and untransfected cells (0.263±0.032

P0.05). Conclusion Transfection of hepaCAM gene significantly inhibits the cell proliferation

and VEGF expressions at mRNA and protein levels in BIU-87 cells. hepaCAM gene might play an important role in the proliferation

invasion and metastasis of bladder cancer．

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