Evodiaminestimulated dentritic cells of spinal cord homogenate protein promotes repair of spinal cord injury in mice

王科; 赵建华; 梁华平

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Evodiaminestimulated dentritic cells of spinal cord homogenate protein promotes repair of spinal cord injury in mice

更新时间：2026-01-09

- Evodiaminestimulated dentritic cells of spinal cord homogenate protein promotes repair of spinal cord injury in mice
- Journal of Army Medical University Vol. 32, Issue 3, Pages: 269-273(2010)
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  CLC： R285.5;R681.54;R392.3
- Published：2010
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王科,赵建华,梁华平.吴茱萸碱刺激的负载脊髓匀浆蛋白DCs促进小鼠脊髓损伤的修复[J].陆军军医大学学报(原第三军医大学学报),2010,32(03):269-273.

　Wang Ke,Zhao Jianhua,Liang Huaping.Evodiaminestimulated dentritic cells of spinal cord homogenate protein promotes repair of spinal cord injury in mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(03):269-273.
王科,赵建华,梁华平.吴茱萸碱刺激的负载脊髓匀浆蛋白DCs促进小鼠脊髓损伤的修复[J].陆军军医大学学报(原第三军医大学学报),2010,32(03):269-273. DOI：

　Wang Ke,Zhao Jianhua,Liang Huaping.Evodiaminestimulated dentritic cells of spinal cord homogenate protein promotes repair of spinal cord injury in mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(03):269-273. DOI：

摘要

目的探讨吴茱萸碱（evodiamine，EVO）刺激的负载脊髓匀浆蛋白（homogenate protein of spinal cord，hp）树突状细胞（hpDCevo）对小鼠脊髓损伤（spinal cord injury，SCI）的促修复作用及其机制。方法 SCI后24 h给予治疗，随机分6组:分别腹腔注射PBS、EVO、未成熟DC（DCs）、经EVO刺激的DCs(DCevo)、负载hp的DC（hpDC）、hpDCevo。利用BMS功能评分及组织病理学方法，观察hpDCevo对小鼠脊髓损伤神经功能恢复和局部瘢痕等病理学改变的影响。ELISA法测定SCI损伤局部组织和T细胞培养上清中的脑源性神经营养因子（brain-derived neurotrophic factor

BDNF）、神经营养因子-3（Neurotrophin-3

NT-3）、白介素-12（interleukin-12

IL-12）、粒-巨噬细胞集落刺激因子（granulocyte-macrophage colony stimulating factor ，GM-CSF）的含量。结果注射后第84天，hpDCevo组BMS评分明显高于hpDC组和其余各组（P＜0.05）。组织病理学结果显示，hpDCevo组较其他组空洞和瘢痕缩小。损伤局部组织中的BDNF、NT-3、GM-CSF、IL-12含量为hpDCevo组高于hpDC组和其余各组（P0.05）；T细胞培养上清中的BDNF、GM-CSF为hpDCevo组高于hpDC组和其余各组（P0.05）。结论 hpDCevo对小鼠SCI修复有促进作用，其机制可能是通过上调损伤局部的BDNF、NT-3、GM-CSF、IL-12来实现的。

Abstract

Objective To study the effect of evodiamine (EVO)-stimulated dendritic cells (DC) of spinal cord homogenate protein (hpDC) on repair of spinal cord injury (SCI) in mice. Methods Twenty hours after SCI

mice were randomly divided into 6 groups and injected with PBS

EVO

DC+EVO

hpDC and hpDC+EVO. BMS and histopathology were used to observe the effect of hpDC+EVO on the functional recovery of spinal cord injury and pathological changes such as local scars. Levels of brain-derived neurotrophic factor (BDNF)

neurotrophin-3 (NT-3)

interleukin-12 (IL-12) and granulocyte macrophage colony stimulating factor (GM-CSF) in injured sites and supernatant of cultured T cells were measured by ELISA. Results Eighty-four days after treatment

the BMS was significantly higher in hpDC + EVO group than in other groups（P0.05）. Histopathology showed that the cavities and scars were smaller in hpDC group than in other groups. The levels of BDNF

NT-3

GM-CSF

and IL-12 in the injured sites were significantly higher in hpDC + EVO group than in other groups（P0.05）. The levels of BDNF and GM-CSF in supernatant of cultured T cells were significantly higher in hpDC + EVO group than in hpDC group（P0.05）. Conclusion hpDC+ EVO promotes the repair of SCI possibly by up-regulating the BDNF

NT-3

GM-CSF

IL-12 in injured sites.

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