Transfection efficiency of recombinant adenovirus Ad-shRNA-NgR in ischemic brain tissues of rats

吴小慧; 秦新月; 王敬; 张沁丽; 李鑫

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Transfection efficiency of recombinant adenovirus Ad-shRNA-NgR in ischemic brain tissues of rats

更新时间：2026-01-09

- Transfection efficiency of recombinant adenovirus Ad-shRNA-NgR in ischemic brain tissues of rats
- Journal of Army Medical University Vol. 32, Issue 8, Pages: 802-804(2010)
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  CLC： R394-33;R394.2;R743.31
- Published：2010
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吴小慧,秦新月,王敬,张沁丽,李鑫.重组腺病毒Ad-shRNA-NgR在大鼠缺血脑组织的转染效率研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):802-804.

　Wu Xiaohui,Qin Xinyue,Wang Jing,Zhang Qinli,Li Xin.Transfection efficiency of recombinant adenovirus Ad-shRNA-NgR in ischemic brain tissues of rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):802-804.
吴小慧,秦新月,王敬,张沁丽,李鑫.重组腺病毒Ad-shRNA-NgR在大鼠缺血脑组织的转染效率研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):802-804. DOI：

　Wu Xiaohui,Qin Xinyue,Wang Jing,Zhang Qinli,Li Xin.Transfection efficiency of recombinant adenovirus Ad-shRNA-NgR in ischemic brain tissues of rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):802-804. DOI：

摘要

目的　探索用于RNA干扰的重组腺病毒Ad-shRNA-NgR转染大鼠缺血脑组织的适宜滴度。　方法　将低、中、高3种不同滴度(7.90×10

、1.58×10

、3.16×10

pfu/ml)的重组腺病毒通过立体定位手术注射到大脑中动脉阻塞（middle cerebral artery occlusion

MCAO）/再灌注模型大鼠（n=10）脑缺血区，于术后48 h和1周在荧光显微镜下观察各组大鼠脑组织中绿色荧光蛋白（green fluorescent protein

GFP）的表达强度并估算转染效率，通过HE染色检测炎性反应。　结果　中滴度和高滴度注射组大鼠脑组织腺病毒转染效率相似，为60%～70%，明显高于低滴度注射组（P0.01）。在各组脑组织切片中，仅高滴度注射组有明显炎症反应，血管周围可见淋巴和巨噬细胞浸润。　结论　中等滴度（1.58×10

pfu/ml）的腺病毒能高效、稳定、低毒地转染大鼠缺血脑组织，推荐用于脑缺血基因干预治疗的实验研究。

Abstract

Objective　To explore a proper titer of RNA interference recombinant adenovirus Ad-shRNA-NgR transfecting ischemic region in rat brains for further study on gene therapy in cerebral ischemia. 　Methods　Three different titers (7.90×10

1.58×10

and 3.16×10

pfu/ml) of recombinant adenovirus were respectively injected into the ischemic region of middle cerebral artery occlusion (MCAO)/reperfusion rats (n=10) by stereotactic surgery. Green fluorescent protein (GFP) expression in each group was observed to measure transfection effic

iency of recombinant adenovirus in postoperative 48 h and 1 week. Inflammation was observed by HE staining. 　Results　Transfection efficiencies of recombinant adenovirus in high and medium titer injection group were similar

about 60% to 70%

both significantly higher than low titer group (P0.01). Besides

inflammation as perivascular infiltration of lymphoid and macrophage was only observed in high titer group. 　Conclusion　Medium titer (1.58×10

pfu/ml) of recombinant adenovirus can stably

with high efficiency and low toxicity

transfect ischemic brain tissue in rats.

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