Objective    To explore the effect of PTEN in the regulation on localization of AMPA recepter subunit GluR2 in rat hippocampal neuron after stretch Injury.      Methods    Cultured the primary rat hippocampal neuron from neonate rats and established the model of stretch injury. Utilized RNAi-lentvirus to inhibit the expression of PTEN gene. Used Tunel dyeing to assess injury of neurons and used Western-blot to investigate localization of AMPA recepter subunit GluR2 in rat hippocampal neuron after stretch Injury.      Results    RNAi-lentvirus can down regulated expression of PTEN gene effectively. There was no evidently difference in cellular apoptotic rates at 0 h

2 h and 6 h

but the celluar apoptotic rate descreased from 68.7%±5.1% to 39.3%±3.5% at 24 h and from 76.7%±5.3% to 52.0%±4.3% at 48 h. AMPA recepter subunit GluR2 descreased evidently at 6 h

24 h and 48 h.  When decresase expression of PTEN

AMPA recepter subunit GluR2 descreased evidently at 24 h and 48 h

but there was no evidently descrease at 6 h after injury. Downregulation on PTEN can obviously increase the AMPA recepter subunit GluR2 at 6 h

24 h and 48 h.      Conclusion    Downregulation of PTEN can inbibit the decrease of AMPA recepter GluR2 subunit in cell membrane of neuron after stretch injury

prevent the apoptosis of neuron. The function of PTEN to AMPA recepter may provides a new way for the prevention and treatment of traumatic brain injury.