Effect of BRL-37344 on ion channel in guinea pig cardiac myocytes

修芸; 孔祥如; 吴兰香

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Effect of BRL-37344 on ion channel in guinea pig cardiac myocytes

更新时间：2026-01-09

- Effect of BRL-37344 on ion channel in guinea pig cardiac myocytes
- Journal of Army Medical University Vol. 32, Issue 11, Pages: 1192-1194(2010)
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  CLC： R322.11;R329.25;R329.27
- Published：2010
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修芸,孔祥如,吴兰香.BRL-37344对豚鼠心肌细胞ICa-L、Ito的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1192-1194.

　Xiu Yun,Kong Xiangru,Wu Lanxiang.Effect of BRL-37344 on ion channel in guinea pig cardiac myocytes[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1192-1194.
修芸,孔祥如,吴兰香.BRL-37344对豚鼠心肌细胞ICa-L、Ito的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1192-1194. DOI：

　Xiu Yun,Kong Xiangru,Wu Lanxiang.Effect of BRL-37344 on ion channel in guinea pig cardiac myocytes[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1192-1194. DOI：

摘要

目的为研究β3肾上腺素能受体介导的心脏生物学效应——β3肾上腺素能受体激活后心肌细胞电生理活动的变化。方法以原代培养的豚鼠心肌细胞为对象

采用全细胞膜片钳技术记录心肌细胞孵育β3肾上腺素能受体激动剂4-[-[2-hydroxy-(3-chlorophenyl)ethyl-amino

]

propyl

]

phenoxyacetate（BRL-37344; BRL）5~10 min前后I

Ca-L

，I

（L型钙离子通道和瞬时外向钾通道）的变化。结果显示10

-6

mol/L的BRL显著增强豚鼠心肌细胞的I

，上移I-V曲线；去极化刺激到+80 mV时，电流密度从（6.11±1.03） pA/pF上升到（8.46±2.07） pA/pF（n=6

P=0.013064）。10

-6

mol/L的BRL显著增强豚鼠心肌细胞的I

Ca-L

，为对照组的（2.30±0.75）倍（n=5，P=0.0063）；去极化刺激到+10 mV时，电流幅度从（89.25±17.83） pA上升到（205.00±72.24） pA。结论 BRL-37344可增强豚鼠心肌瞬时外向钾电流和L型钙电流，进而调控心脏的活动。

Abstract

Objective To study the biological effect of β3 adrenoceptoractivated electrophysiological activities in cardiac myocytes. Methods Changes of transient outward potassium (I

) and L-type calcium current (I

Ca-L

) in primary cultured guinea pig cardiacmyocytes were detected with the standard whole-cell patch clamp technique about 5-10 min before and after administration of β3 adrenoceptor agonist

4-[-[2-hydroxy-(3-chlorophenyl)ethyl-amino

]

propyl

]

phenoxyacetate

(BRL-37344; BRL). Results BRL could significantly increased the I

in guinea pig cardiac myocytes with the I-V curve up-moved. When the membrane potential reached to +80 mV

the density current increased from 6.11±1.03 pA/pF to 8.46±2.07 pA/pF (n=6

P=0.013 064). BRL (10

-6

mol/L) could significantly increase the I

Ca-L

in guinea pig cardiac myocytes

which was (2.30±0.75)fold higher than in control group（n=5，P=0.0063）. When the membrane potential increased to +10 mV

the current

increased from 89.25±17.83 pA to 205.00±72.24 pA （n=5，P=0.006 3）. Conclusion BRL-37344 can increase the transient I

and I

Ca-L

thus further regulating the cardiac activities.

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