Hypoxia induces expression of interleukin-1 receptor-associated kinases-1 in rat neurons

李江涛; 胡胜利; 杜鹏; 郑江; 缪洪平; 吴国材; 唐卫华; 刘智; 朱刚

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Hypoxia induces expression of interleukin-1 receptor-associated kinases-1 in rat neurons

更新时间：2026-01-09

- Hypoxia induces expression of interleukin-1 receptor-associated kinases-1 in rat neurons
- Journal of Army Medical University Vol. 32, Issue 8, Pages: 794-797(2010)
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  CLC： R331.8;R345;R364.4
- Published：2010
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李江涛,胡胜利,杜鹏,郑江,缪洪平,吴国材,唐卫华,刘智,朱刚.缺氧条件下大鼠神经元白细胞介素-1受体相关激酶-1的表达变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):794-797.

　Li Jiangtao,Hu Shengli,Du Peng,Zheng Jiang,Miao Hongping,Wu Guocai,Tang Weihua,Liu Zhi,Zhu Gang.Hypoxia induces expression of interleukin-1 receptor-associated kinases-1 in rat neurons[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):794-797.
李江涛,胡胜利,杜鹏,郑江,缪洪平,吴国材,唐卫华,刘智,朱刚.缺氧条件下大鼠神经元白细胞介素-1受体相关激酶-1的表达变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):794-797. DOI：

　Li Jiangtao,Hu Shengli,Du Peng,Zheng Jiang,Miao Hongping,Wu Guocai,Tang Weihua,Liu Zhi,Zhu Gang.Hypoxia induces expression of interleukin-1 receptor-associated kinases-1 in rat neurons[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):794-797. DOI：

摘要

目的　观察大鼠神经元B35细胞株中白细胞介素-1受体相关激酶-1（interleukin-1 receptor-associated kinases-1，IRAK-1）mRNA及蛋白在缺氧后表达量的变化，并探讨其变化与神经元中TNF-α表达的关系。　方法　将培养的B35细胞置于低氧（3%O

、5%CO

、92%N

）条件下培养1、3、6、12、24、48、72 h，应用RT-PCR方法检测IRAK-1 mRNA表达的变化；Western blot检测IRAK-1蛋白含量的变化；激光扫描共聚焦观察IRAK-1在细胞内的分布及含量变化；酶联免疫吸附法检测培养液上清肿瘤坏死因子-α（TNF-α）的含量，并与IRAK-1蛋白进行相关分析。　结果　B35细胞IRAK-1 mRNA表达与正常对照相比，1 h开始增加[（0.786±0.042），P0.05

]

，3 h增加明显[（0.947±0.055），P0.05

]

，6 h达到高峰（1.245±0.077），P0.05

]

，12 h仍维持在较高水平[（0.911±0.041），P0.05

]

，24 h降至正常水平以下但无明显差异[（0.596±0.047），P0.05

]

，48 h以后进一步减少[（0.520±0.049），P0.05

]

。IRAK-1蛋白表达变化也呈类似趋势。相关性分析结果显示，培养上清液中TNF-α含量的变化与IRAK-1蛋白表达变化呈正相关（r=0.88，P0.05）。　结论　神经元内存在TLR/IL-1R家族信号转导系统的关键因子IRAK-1，缺氧损伤可诱导其表达，与TNF-α的表达相关。

Abstract

Objective　To investigate the dynamical changes of interleukin-1 receptor-associated kinases-1 (IRAK-1) mRNA and protein

and to explore the relationship between these changes and concentrations of TNF-α in rat B35 neurons under hypoxia. 　Methods　B35 cells exposed to hypoxia of 3%O

5%CO

and 92%N

were cultured for

48 and 72 h respectively

the mRNA and protein expressions of IRAK-1 were detected by RT-PCR and Western blot analysis

the distribution of IRAK-1 in the cells were observed by laser scanning confocal microscopy (LSCM)

and the concentrations of TNF-α were measured by ELISA. 　Results　The mRNA and protein expressions of IRAK-1 were increased after hypoxia for 1 h and decreased gradually after reaching the highest level at 6 h (P0.05)

so did the fluorescence intensity under LSCM and the concentrations of TNF-α. After 48 h

the concentrations of mRNA and protein were decreased lower than that in normoxia (P0.05). The concentrations of TNF-α were correlated positively with the expression of IRAK-1 protein (r=0.88

P0.05). 　Conclusion　Hypoxia up-regulates the mRNA and protein of IRAK-1

which are correlated positively with the expression of TNF-α in rat B35 neurons.

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