Objective   To investigate the expression of heat shock protein (HSP) 60 and analyze the significance in cervical carcinoma.    Methods   The cervical tissue samples of 57 cases of cervical cancer and 57 normal cervical tissues were collected. The expression of HSP60 were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The protein expressions of HSP60 were determined by Western blot analysis. To observe the relationship between HSP60 expression and the occurrence and development of cervical cancer.    Results   HSP60 mRNA expression in normal cervical tissue was significantly lesser than that in cervical cancer tissue (t=2.65

P0.05). The positive rate of HSP60 expression in cervical cancer and normal cervix was 82.5% and 19.3% respectively. The over-expression rate of HSP60 was 57.9% and 7.0% respectively. The difference of HSP60 expression had no significance at different stages of cervical cancer tissue (P0.05). The levels of HSP60 protein expression in normal cervical tissue was significantly lower than the expression in cervical cancer tissue (t=3.132

P0.05).     Conclusion   The expression of HSP60 is closely related to the  incidence of cervical cancer

but there is no relationship with different stages of cervical cancer.