Liang Yufen,Wei Junbin,Yun Chenxia,Huang Shushi.Five isozymograms of Trichomonas vaginalis and Trichomonas tenax: A comparative study[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1180-1182.
Liang Yufen,Wei Junbin,Yun Chenxia,Huang Shushi.Five isozymograms of Trichomonas vaginalis and Trichomonas tenax: A comparative study[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1180-1182.DOI:
Five isozymograms of Trichomonas vaginalis and Trichomonas tenax: A comparative study
Objective To analyze the characteristics of isozymograms of Trichomonas vaginalis (TV) and Trichomonas tenax (TT) and their relation in an attempt to find the identification index of their isozymograms. Methods Five isozymes including melate dehydrogenase (MDH)
glutamic dehydrogenase (GLDH)
glucose-6-phosphate dehydrogenase (G6PD)
alcohol dehydrogenase (ADH) and catalase were stained with SDS-PAGE. Isozymograms of TV and TT were compared and analyzed. Results Two identical bands (Rm52 and Rm85) of MDH isozymogram and 4 bands (Rm34
Rm42
Rm94 and Rm100) of MDH isozymogram were found in TV and TT
respectively. No band and 6 band of GLDH isozymogram were identified in TV and TT
respectively. Three bands (Rm35
Rm42 and Rm52) of G6PD isozymogram were observed in TV
and more bands of G6PD isozymogram were demonstrated in TT
in addition to the 3 bands in TV. No band of ADH and catalase isozymogram was displayed in TV and TT. Conclusion MDH
GLDH and G6PD can be used as an identification index of TV and TT isozymograms.