Objective  To investigated the effect of somatostatin (SS) on cholecystokinin-induced alterations of exocrine function and the redistribution of the F-actin in the pancreatic acinar cells.    Methods  In vitro

isolated rat pancreatic acini were divided into 12 groups: normal control group

different concentrations of cholecystokinin-octapep

tide groups (CCK-8) of 10

-12

10

-11

10

-10

10

-9

or 10

-8

mol/L

SS group (10

-7

mol/L)

SS and different dose CCK-8 groups. The acinar cells were stimulated with different concentrations of CCK-8 for 30 min in the presence or absence of 10

-7

mol/L SS. Amylase and trypsin activity were measured using corresponding assay kits

and F-actin distribution in pancreatic acinar cells were detected by laser confocal microscopy.   Results  SS stabilized the structure of cytoskeleton in acinar cells

and remarkably increased the ratio of subapical/basolateral F-actin (P0.05); Exposure to low concentrations of CCK-8 (10

-11

to 10

-10

mol/L) in the presence of SS

the CCK-induced amylase release were significantly inhibited (P0.05). However

at higher concentrations of CCK-8 (10

-9

to 10

-8

mol/L)

the CCK-induced inhibition of amylase release was not observed as SS present

and SS significantly inhibited CCK-stimulated trypsin activity in acinar cells (P0.05).   Conclusion  SS stabilizes the cytoskeleton morphology in the acinar cells

and prevents the trypsinogen activation

which might be one of the protective mechanisms of SS on CCK-induced acute injury in acinar cells.