Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice

魏秀丽; 黄志; 李欣; 刘玮

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Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice

更新时间：2026-01-09

- Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice
- Journal of Army Medical University Vol. 32, Issue 4, Pages: 353-356(2010)
- 作者机构：
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  CLC： R593.202;R392.12;R-332
- Published：2010
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魏秀丽,黄志,李欣,刘玮.Foxp3基因及调节性T细胞在重症肌无力被动转移幼鼠发病中的作用机制[J].陆军军医大学学报(原第三军医大学学报),2010,32(04):353-356.

　Wei Xiuli,Huang Zhi,Li Xin,Liu Wei.Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(04):353-356.
魏秀丽,黄志,李欣,刘玮.Foxp3基因及调节性T细胞在重症肌无力被动转移幼鼠发病中的作用机制[J].陆军军医大学学报(原第三军医大学学报),2010,32(04):353-356. DOI：

　Wei Xiuli,Huang Zhi,Li Xin,Liu Wei.Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(04):353-356. DOI：

摘要

目的探讨Foxp3

CD4

CD25

调节性T细胞、Foxp3 mRNA在重症肌无力被动转移模型(passive transferred myasthenia gravis，PTMG)发病中的作用机制。方法将16只幼年雌性C57BL/6小鼠随机分为2组：模型组和对照组，模型组经腹腔注射含1.0 mg/kg mAb35的Ringer’s液0.2 ml建立PTMG模型，正常对照组注射不含mAb35的Ringer’s液0.2 ml。采用流式细胞技术检测小鼠脾细胞中CD4

CD25

T细胞及Foxp3

CD4

CD25

Tregs含量，实时荧光定量聚合酶链反应(RT-FQ-PCR)分析2组小鼠脾细胞中Foxp3 mRNA的表达， ELISA法检测2组小鼠血清白介素-2和干扰素-γ的水平。 结果 ①模型组小鼠脾细胞中CD4

CD25

T细胞与CD4

T细胞含量的比例与对照组比较有统计学差异[(8.82±0.74)％vs (9.89±0.88)％，P0.05

]

；模型组Foxp3

CD4

CD25

Tregs与CD4

CD25

T细胞含量的比例与对照组比较显著降低[(6.83±1.18)％ vs (8.38±0.76)％，P0.01

]

；脾细胞悬液中Foxp3 mRNA表达量模型组低于对照组[(0.47±0.30) vs (1.53±1.19)，P0.05

]

；模型组小鼠血清IL-2、IFN-γ水平分别为(24.41±13.83) ng/L、(142.31±6.05) ng/L，高于对照组小鼠[(12.09±2.96) ng/L、(109.36±3.64) ng/L

]

（P0.05）。②模型组小鼠Foxp3

CD4

CD25

Tregs数量变化与CD4

CD25

T细胞数量呈正相关（r=0.864，P0.01），对照组小鼠Foxp3

CD4

CD25

Tregs数量变化与CD4

CD25

T细胞数量亦呈正相关（r=0.977，P0.01）。结论 Fox

p3 mRNA表达下调

导致Foxp3

CD4

CD25

Tregs细胞亚群表达降低，并通过上调的IL-2和IFN-γ介导了Foxp3及Tregs在重症肌无力发病机制中的作用。

Abstract

Objective To study the role of Foxp3

CD4

CD25

regulatory T cells (Foxp3

CD4

CD25

Tregs) and Foxp3mRNA in pathogenesis of passive transferred myasthenia gravis (PTMG) by detecting their expression in a PTMG model. Methods Sixteen C57BL/6 young female mice were divided into model group and control group. A PTMG model was established by injecting Ringer’s solution containing 1.0 mg/kg mAb35 into abdominal cavity. Mice in control group were injected with Ringer’s solution not containing mAb35. Levels of CD4

CD25

T cells and Foxp3

CD4

CD25

Tregs in spleen cells were measured by flow cytometry. Expression of Foxp3 mRNA in spleen cells was detected by real-time fluorescent quantitative polymerase chain reaction (RT-FQ-PCR). Serum level of interleukin-2 and interferon-γ was detected by ELISA. Results The ratio of CD4

CD25

T cells and CD4

T cells was higher in model group [(8.82±0.74)%

]

than in control group [(9.89±0.88)%

]

(P0.05). The ratio of Foxp3

CD4

CD25

Tregs and CD4

CD25

T cells was significantly lower in model group [(6.83±1.18)%

]

than in control group [(8.38±0.76)%

]

(P0.01). The expression level of Foxp3 mRNA in spleen cells was significantly lower in model group (0.47±0.30) than in control group (1.53±1.19) (P0.05). The serum level of IL-2 and IFN-γ was higher in model group (24.41±13.83 and 142.31±6.05 ng/L) than in control group (12.09±2.96 and 109.36±3.64 ng/L) (P0.05）. The change in the number of Foxp3

CD4

CD25

Tregs was positively correlated with that of CD4

CD25

T cells between model and control groups（r=0.864 vs r=0.977，P0.01). Conclu

sion Down-regulation of Foxp3 expression can decrease the expression of Foxp3

CD4

CD25

Tregs subgroups

and upregulation of IL-2 and IFN-γ plays an important role in the pathogenesis of Foxp3-mediated PTMG.

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