Role of parathyroid hormone-related peptide in transdifferentiation of NRK-52E cells induced by high glucose

梁华晟; 钟宇华

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Role of parathyroid hormone-related peptide in transdifferentiation of NRK-52E cells induced by high glucose

更新时间：2026-01-09

- Role of parathyroid hormone-related peptide in transdifferentiation of NRK-52E cells induced by high glucose
- Journal of Army Medical University Vol. 32, Issue 11, Pages: 1165-1168(2010)
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  CLC： R322.61;R394.1;R587.102
- Published：2010
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梁华晟,钟宇华.甲状旁腺激素相关肽在高糖诱导NRK-52E细胞转分化中的作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1165-1168.

　Liang Huasheng,Zhong Yuhua.Role of parathyroid hormone-related peptide in transdifferentiation of NRK-52E cells induced by high glucose[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1165-1168.
梁华晟,钟宇华.甲状旁腺激素相关肽在高糖诱导NRK-52E细胞转分化中的作用[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1165-1168. DOI：

　Liang Huasheng,Zhong Yuhua.Role of parathyroid hormone-related peptide in transdifferentiation of NRK-52E cells induced by high glucose[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1165-1168. DOI：

摘要

目的探讨甲状旁腺素相关肽在高糖诱导大鼠肾小管导管上皮细胞株NRK-52E转分化作用。方法应用RT-PCR及Western blot检测无糖组（无糖培养基培养），正常葡萄糖组（含5 mmol/ L葡萄糖DMEM培养基培养）组及高浓度葡萄糖组（含16.7 mmol/L葡萄糖DMEM培养基培养）中甲状旁腺素相关肽（parathyroid hormone-related peptide

PTHrP）及其受体1在大鼠肾小管导管上皮细胞株NRK-52E表达。10 pmol/L PTHrP及16.7 mmol/L葡萄糖分别干预72 h，应用Western blot检测转化因子β、金属蛋白酶2、Ⅰ型胶原及α平滑肌肌动蛋白表达情况。ROS含量应用CM2H2DCFDA试剂盒检测。结果与空白组和正常葡萄糖组相比，高浓度葡萄糖能显著上调PTHrP(P0.05

P0.01)和甲状旁腺激素受体1(Type 1 receptor parathyroid hormone-related protein，PTH1R) (P0.01

P0.05)蛋白及 PTHrP mRNA(P0.01)表达，PTH1R mRNA在高浓度及正常葡萄糖浓度组的表达高于空白组(P0.01)。对比空白组，PTHrP能显著上调NRK-52E细胞转化因子β1(P0.01)、Ⅰ型胶原(P0.01)及α平滑肌肌动蛋白(P0.01)表达，而在高浓度葡萄糖状态下，其表达上调更显著(P0.05

P0.01)。金属蛋白酶2在PTHrP及高浓度葡萄糖共同干预下表达上调(P0.01)。PTHrP及高血糖均可以升高NRK-53E细胞ROS含量(P0.01

P0.01)。结论高浓度葡萄糖可能通过调控PTHrP/PTH1R的水平从而影响肾小管导管上皮转分化。

Abstract

Objective To study the role of parathyroid hormone-related peptide (PTHrP) in transdifferentiation of NRK-52E cells induced by high glucose. Methods Expression of PTHrP and its receptor 1 in NRK-52E cells incubated with 0 (control group)

5 (normal glucose group) and 16.7mmol/L glucose (high glucose group)

respectively

was detected by RT-PCR and Western blotting

respectively. Expression of TGF-β1

MMP2

type Ⅰ collagen and α-SMA in NRK-52E cells of normal and high glucose groups was detected by Western blotting and ROS was detected by CM2H2DCFDA after 72 h. Results The expression levels of PTHrP

PTHrP receptor 1 (PTH1R)

and PTHrP mRNA were higher in high glucose group than in control and normal glucose groups (P0.05). The expression of TGF-β1

type Ⅰ collagen

and α-SMA after interfered with PTHrP was significantly higher in normal and high glucose groups than in control group (P0.05). The expression of MMP2 was up-regulated after interference with PTHrP and high glucose level (P0.01). The PTHrP and high glucose level increased the ROS in NRK-52E cells (P0.01). Conclusion High glucose level can influence the transdifferentiation of NRK-52E cells by regulating PTHrP and its receptor level.

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