Objective   To study the slow-release properties of amphotericin B liposome(AmBL)-fibrin glue(FG)-amniotic membrane（AFAM）in vivo and in vitro.    Methods   AmBL was added into FG and cross-linked with AM to prepare the AFAM. Morphology of the AFAM was observed under light microscope and scanning electron microscope (SEM). AFAM was placed in saline which was replaced once a day for 10 d. Concentration of the released AmB was assayed by high performance liquid chromatography (HPLC). AFAM was transplanted to the right eyes of rabbits

and changes of AmB concentration in aqueous humor were observed. AmBL solution was dropped into the left eyes which served as controls.    Results   The AmBL-FG was firmly fixed on the basal surface of amniotic membrane. AmBL was attached to the FG meshes. The concentration of AmB in saline was 59.9

34.1 μg/ml

and 27.9 μg/ml respectively in the initial 3 days and 0.78 μg/ml on day 10

which was then gradually reduced. After transplantation of AFAM

the AmB concentration in aqueous humor was 113.1 and 61.2 ng/ml respectively in the first 2 days

which was then gradually reduced. The AmB concentration in aqueous humor of the control group was relatively low with a mean level of 26.1 ng/ml.    Conclusion   AFAM is a rather good slow-release system of drugs.