A tissue engineering bone to repair femoral head necrosis

彭吾训; 王蕾; 邓进; 李彦林; 龚跃昆; 李世和

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A tissue engineering bone to repair femoral head necrosis

更新时间：2026-01-09

- A tissue engineering bone to repair femoral head necrosis
- Journal of Army Medical University Vol. 32, Issue 7, Pages: 661-664(2010)
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  CLC： R318.08;R681.805;R-332
- Published：2010
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彭吾训,王蕾,邓进,李彦林,龚跃昆,李世和.一种组织工程骨修复兔股骨头坏死模型的效果评价[J].陆军军医大学学报(原第三军医大学学报),2010,32(07):661-664.

　Peng Wuxun,Wang Lei,Deng Jin,Li Yanlin,Gong Yuekun,Li Shihe.A tissue engineering bone to repair femoral head necrosis[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(07):661-664.
彭吾训,王蕾,邓进,李彦林,龚跃昆,李世和.一种组织工程骨修复兔股骨头坏死模型的效果评价[J].陆军军医大学学报(原第三军医大学学报),2010,32(07):661-664. DOI：

　Peng Wuxun,Wang Lei,Deng Jin,Li Yanlin,Gong Yuekun,Li Shihe.A tissue engineering bone to repair femoral head necrosis[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(07):661-664. DOI：

摘要

目的观察双相陶瓷生物骨(biphasic ceramic biologic bone，BCBB)、骨形态发生蛋白(bone morphogenetic protein，BMP)、碱性成纤维细胞生长因子(basic fibroblast growth factor，bFGF)、骨髓基质干细胞(mesenchymal stem cells，MSCs)复合培养形成的组织工程骨修复股骨头坏死(femoral head necrosis，FHN)模型病灶清除区缺损，评价该组织工程骨修复FHN的效果。方法取32只新西兰成年大白兔共64侧股骨头，微波灭活建立股骨头坏死模型，按随机数字表法分为4组，每组16侧。A组：空白对照组；B组：植入BCBB /BMP/bFGF；C组：植入BCBB/BMP/bFGF/MSCs；D组：植入自体松质骨。术后2、4、8、12周分批处死动物，每组每次处死4只。行股骨头大体及解剖观察、X线摄片、组织学观察、钙含量及钙磷比检测、血管免疫组织化学染色。结果 ①股骨头大体及解剖观察：A组8周和12周各有一侧股骨头塌陷；B组2周时纤维结缔组织生长活跃；C、D组12周时植骨区与宿主骨界限不清。②X线评分：12周时，B、C、D组优于A组（P0.05），但B、C、D组间无统计学差异（P0.05）。③组织学新骨形成面积比较：4、8、12周时C组优于B组（P0.05），C、D组间无统计学差异（P0.05）。④X射线能谱分析：2、4、8周时B、C组钙含量及钙磷比显著大于D组（P0.05）。⑤2、4 周和8周时，B、C组血管面积大于A、D组，差异具有统计学意义（P0.05）。结论组织工程骨BCBB/BMP/bFGF/MSCs对兔股骨头坏死的修复效果优于BCBB/BMP/bFGF，与自体松质骨移植相当。

Abstract

Objective To explore the efficacy of a tissue engineering bone composed of biphasic ceramic biologic bone (BCBB)

bone morphogenetic protein (BMP)

basic fibroblast growth factor (bFGF) and mesenchymal stem cells (MSCs) in the repairing of necrotic areas of the femoral head. Methods The femoral head necrosis model of 64 femoral head in 32 rabbits was induced by microwave heating and then randomly divided into 4 groups

which were respectively implanted with nothing (group A)

with BCBB/BMP/bFGF (group B)

with BCBB/BMP/bFGF/MSCs (group C) and with cancellous bone autograft (group D). The specimens were harvested separately at the end of 2

8 and 12 weeks after operation. Four femoral heads were taken off at each interval in every group. A series of examinations were carried out including of gross anatomic observation

X-ray

histology

the calcium content and the ratio of calcium and phosphorus

and blood vessel immunohistochemical staining. Results (1) Gross anatomic observation: In group A

one femoral head collapsed at the end of 8 and 12 weeks respectively. In group B

granulation and fibrillar tissue began to proliferate actively by the end of 2 weeks. The boundary between the grafting substance and the post bone was unclear in group C and group D by the end of 12 weeks. (2) X-ray: At the 12th week

X-ray grade point indicated that group B

D was superior to group A (P0.05)

and among group B

and D

the difference was not significant (P0.05). (3) Histology: New bone area comparison by the end of 4

8 and 12 weeks showed Group C was superior to group B (P0.05)

and the difference between group C and D was not significant (P0.05). (4) Energy dispersion analysis X-ray (EDAX): The calcium content and the ratio of calcium and phosphorus in group B and group C was higher than that of group D at the 2nd

4th

and 8th weeks (P0.05). (5) At the 2nd

4th and 8th week

the area of new blood vessel in group B and C was more than that of group A and D

and the difference was obvious (P0.05). Conclusion The tissue engineering bone (BCBB/BMP/bFGF/MSCs) is superior to BCBB/BMP/bFGF

and comparable to cancellous bone autograft in repairing rabbit femoral head necrosis.

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