Microinjection of adenosine into orexinergic zone of lateral hypothalamus on sleep-wake cycle in rats

寸艳萍; 夏建霞; 李洋; 胡志安

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Microinjection of adenosine into orexinergic zone of lateral hypothalamus on sleep-wake cycle in rats

更新时间：2026-01-09

- Microinjection of adenosine into orexinergic zone of lateral hypothalamus on sleep-wake cycle in rats
- Journal of Army Medical University Vol. 32, Issue 8, Pages: 767-769(2010)
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  CLC： R338.24;R338.63;R977.4
- Published：2010
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寸艳萍,夏建霞,李洋,胡志安.外侧下丘脑orexin能神经元区微注射腺苷对大鼠睡眠-觉醒周期的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):767-769.

　Cun Yanping,Xia Jianxia,Li Yang,Hu Zhian.Microinjection of adenosine into orexinergic zone of lateral hypothalamus on sleep-wake cycle in rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):767-769.
寸艳萍,夏建霞,李洋,胡志安.外侧下丘脑orexin能神经元区微注射腺苷对大鼠睡眠-觉醒周期的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):767-769. DOI：

　Cun Yanping,Xia Jianxia,Li Yang,Hu Zhian.Microinjection of adenosine into orexinergic zone of lateral hypothalamus on sleep-wake cycle in rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):767-769. DOI：

摘要

目的　探讨外侧下丘脑orexin能神经元区微注射腺苷对SD大鼠睡眠-觉醒周期的影响。　方法　选用成年雄性SD大鼠14只，按3种不同的给药模式在外侧下丘脑orexin能神经元区分别微注射1、10、20 nmol/μl腺苷，以人工脑脊液为自身对照，然后采用脑电机同步记录大鼠的脑电和肌电活动，观察外侧下丘脑区微注射腺苷后大鼠睡眠-觉醒周期的变化。　结果　与人工脑脊液自身对照相比，在大鼠外侧下丘脑分别微注射1、10 nmol/μl和20 nmol/μl腺苷后的3 h内，大鼠觉醒时间明显减少到人工脑脊液自身对照的84%、62%和60%，并且大鼠睡眠时间均有不同程度的延长（P0.05）。在微注射20 nmol/μl腺苷后，大鼠觉醒时间持续减少了3 h，并且与微注射1、10 nmol/μl腺苷后相比，其觉醒时间的减少和NREM/REM睡眠时间的增加也最明显。　结论　在外侧下丘脑orexin能神经元区微注射腺苷促进大鼠睡眠。

Abstract

Objective　To investigate the effect of extragenous adenosine (AD) by microinjection in the orexinergic area of rat lateral hypothalamus (LH) on the sleep-wake cycle. 　Methods　A total of 14 male SD rats were given 1

10 or 20 nmol AD and artificial cerebrospinal fluid (aCSF) as own control through three different models of microinjections into the LH. Their electroencephalogram (EEG) and electromyogram (EMG) were recorded by a polygraph recording instrument to observe the changes of sleep-wake after microinjections. 　Results　AD administration to LH at doses of 1

10 and 20 nmol decreased the total amount of wakefulness by 84%

62% and 60% fold during that first 3-hour recording period and concomitantly increased non rapid eye movement (NREM) and REM sleep

when compared with aCSF treatment. The sleep-inducing effect was maintained even in 3 hour after the 20 nmol AD microinjection. The maximal sleep-inducing effect was obtained at the 20 nmol dose of AD administration into the orexinergic LH (P0.05). 　Conclusion　Unilateral microinjection of AD into orexinergic zone of LH promotes NREM/REM sleep of rats.

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