Objective  To establish a simultaneous method for determination of 5 constituents from Panax quinquefolium stem leaf 20(S)-protopanaxdiol saponins

Ginsenoside-Rb

1

Rc

Rb

2

Rb

3

and Rd by RP-HPLC．  Methods  Panax quinquefolium stem leaf 20(S)-protopanaxdiol saponins was provided by the Ji’an Yisheng Pharmaceutical Stock Co. Ltd.. The Agilent ODS column (5 μm

4.6 mm×250 mm) was used

and eluted with acetonitrile-0.2% phosphoric acid water in gradient mode. The UV detection wavelength was 203 nm

and the flow rate was 1.0 ml/min

with the temperature of column at 40 ℃. The accuracy

stability and repeability of this method was

evaluated. The yield rate was measured.   Results  Five compounds were baseline separated

with good linearity. A standard curve was plotted. Our method was proved to have sound accuracy

stability and repeability. The yield rate was 98.39%

97.36%

98.02%

97.70% and 97.01% respectively for Ginsenoside-Rb

1

Rc

Rb

2

Rb

3

and Rd.   Conclusion  Our method of determination is rapid and accurate

and can be used for controlling the quality of Panax quinquefolium 20(S)-protopanaxdiol saponins.