Objective  To construct the prokaryotic expression plasmid of bcfD gene of Salmonella enterica (S. enterica) serovar typhi and to express BcfD protein.    Methods  The bcfD gene of S. enterica serovar typhi strain ty2 was amplified by PCR and subcloned into procaryotic expression vector pET-30a. The recombinant plasmid pET-30a-bcf was transformed into E.coli BL21 (DE3) and its procaryotic expression was induced by IPTG. The expressed protein was analyzed by SDS-PAGE and Western bloting.    Results  The DNA segment was amplified to the expected size of bcfD gene which was confirmed by bcfD gene sequencing. SDS-PAGE showed that the BcfD protein was expressed as inclusion body with a molecular weight of about 42 kDa. Western blot analysis showed immune reaction of the expressed product with the typhoid sera treated with absorbent E.coli BL21(DE3).    Conclusion  The prokaryotic expression vector of bcfD gene we constructed can express the target protein in E.coli BL21 (DE3).